FastQCFastQC Report
Sun 21 May 2023
EGAF00007935591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478661
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA51161.0688148815132212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29200.6100350770169285No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27480.5741015039871642No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA25540.5335717762675464No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT22560.47131477183225706No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC19100.39902979352819634No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16700.34888992418433923No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16590.34659184683941247No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC16200.33844411807103564No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12550.2621897334439196No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA10660.2227045863356321No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10020.20933395451060355No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9490.19826140003050174No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9440.19721681941917138No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9030.18865125840626248No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT8820.18426401983867496No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA8460.1767430394370964No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT7760.16211891087847138No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA7420.15501576272142498No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7220.15083744027610355No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7140.14916611129797497No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7100.1483304468089107No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7080.14791261456437854No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG6860.143316459874525No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC6830.14268971150772677No Hit
CAGACTGTTACCTCTACCCCGGTCCAGGGCC6790.14185404701866247No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6750.1410183825295982No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6400.1337063182502857No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6360.13287065376122142No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6160.12869233131589997No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6120.1278566668268357No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG6070.12681208621550533No Hit
CCGGCTGCCTCCCCGCCGCGTACCCCTGCCT6030.12597642172644105No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6020.125767505604175No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5900.12326051213698212No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5720.11950002193619284No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5700.11908218969166068No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5500.11490386724633928No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5340.11156120929008213No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5250.10968096418968748No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5070.10592047398889819No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC5070.10592047398889819No Hit
CGTTACGATGACTTCACCTTTGAAGCCGGTA4890.10215998378810892No Hit
GAATGCGACAACTATCGCCAGGAATACGATA4880.10195106766584285No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC4850.10132431929904463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)