FastQCFastQC Report
Wed 31 May 2023
EGAF00007935748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458642
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT64711.4109043654964004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43550.9495423445737634No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC29570.6447294403914164No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG23820.5193593260102651No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20560.4482799220306906No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG19840.4325814033603551No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18290.3987859812228274No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16200.35321667008254803No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG15700.3423149210059262No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG15000.3270524722986556No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT14640.31920321296348786No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA13980.304812904182347No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12840.2799569162876492No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12140.26469446758037857No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11980.26120590787585957No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11790.2570632432267433No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA11440.249432018873108No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG11380.24812380898391337No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11210.24441721429786195No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG8950.19514130847153116No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8260.18009689474579302No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8020.17486405518901452No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7930.1729017403552226No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7890.17202960042909282No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7680.16745086581691165No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7580.1652705160015873No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG7100.15480483688803032No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG7060.15393269696190057No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC6980.15218841710964107No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6930.15109824220197887No Hit
CATTCTAACGATCAGCACGCGGGTGATCACA6330.13801614331003267No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5950.12973081401180006No Hit
GCTATGCAGACCGGTTGGTACAAAGTAGGCA5620.12253565962122963No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5580.12166351969509989No Hit
AGCAACAAAACCGAAACTCCGTCCGAGACTG5430.11839299497211334No Hit
AGCACCCCGCGTGCGGCTGTCACCCAGACGG5270.11490443526759432No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5270.11490443526759432No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5250.11446836530452946No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5020.1094535607292834No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4870.10618303600629685No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4770.10400268619097248No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4630.10095019644951837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)