FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007935766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522340
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT87001.6655818049546274No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC39780.7615729218516675No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC32390.6200941915227629No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC22580.4322854845502929No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20980.40165409503388594No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT20200.38672129264463756No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT17340.33196768388406017No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC17020.32584140598077876No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC17000.3254585136118237No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC16310.31224872688287325No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC14430.2762568442010951No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA14320.2741509361718421No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA14080.26955622774438104No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA13340.25538921009304283No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC13110.25098594785005934No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC12900.24696557797603097No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12730.24371099283991268No Hit
AGCATCCTGCAGCAACTGAACCCGTGTAAAG12720.24351954665543513No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA12640.24198797717961482No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11730.22456637439215837No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT11710.22418348202320326No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA11690.2238005896542482No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG11550.22112034307156261No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT11500.2201631121491749No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG11270.2157598499061914No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG11020.2109736952942528No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT10610.20312440173067353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10210.19546655435157176No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9960.1906803997396332No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC9550.18283110617605391No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9300.17804495156411532No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9270.1774706130106827No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9120.17459892024351956No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC9070.17364168932113183No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC9020.1726844583987441No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG8990.1721101198453115No Hit
GTCGTGCAGCCGTCTATCCTGCAGCAGCTGA8170.15641153271815292No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT8150.15602864034919783No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8140.1558371941647203No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG8060.15430562468889997No Hit
GGTCGTAAAGTACGCGGTGTCATTCCGACTG8030.15373128613546733No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC7620.14588199257188803No Hit
GAGCAGATCCGTCACGGTGATAACGGCGGTG7610.1456905463874105No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7430.14224451506681474No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7380.141287284144427No Hit
TCTATCCTGCAGCAGCTGAACCCGTGCAAAG7230.13841559137726384No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6930.13267220584293754No Hit
ATTCCGGTAGTCCAGCCGTCTATCCTGCAGC6900.1320978672895049No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6750.12922617452234178No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6660.12750315886204389No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6560.12558869701726844No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT6550.1253972508327909No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6530.12501435846383582No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA6390.1223341118811502No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT6340.12137688095876248No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6340.12137688095876248No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6160.11793084963816672No Hit
TCCGTCAACGGCCAGGCCAAATTCGGCGGCA6160.11793084963816672No Hit
GCGTCTGAGCTGGCCAAAGAAGGTAAAACCA6160.11793084963816672No Hit
CAACAGTACCAGCAGGTGCTGCAGCAGCAGA6140.11754795726921163No Hit
GCCCCGTTCGACACCTTCACCGGTGACTACG6100.11678217253130146No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6100.11678217253130146No Hit
CCAGTTGTGCAGCCGTCCATCCTGCAGCAAC6080.11639928016234635No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5870.11237891028831795No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5840.11180457173488532No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5810.1112302331814527No Hit
ATGCTGGTGCAAGTACAGATCCCGTTCGTTC5710.10931577133667725No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC5640.10797564804533445No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC5620.10759275567637937No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5600.10720986330742428No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5510.1054868476471264No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5490.10510395527817132No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5490.10510395527817132No Hit
AAATCTGGCATGGAATCCCTGAAAAACATTC5470.10472106290921623No Hit
GGCGGTACCTACCGTAGCTATGAACAGCTGA5350.1024237086954857No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5260.10070069303518782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)