FastQCFastQC Report
Sun 21 May 2023
EGAF00007935768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498392
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT81431.6338544759947993No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49690.9970063724939405No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC39500.7925488370599849No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC30390.6097609913481757No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC20590.4131286216472175No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20210.40550410118942515No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT18790.37701247211030675No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT16780.3366827717940898No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC16130.3236408289057609No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC16070.3224369572545306No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC15930.31962792340165974No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC13700.27488402703093145No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC13560.27207499317806066No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA13290.26665757074752405No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13280.266456925472319No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC12370.24819820542865859No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12130.24338271882373713No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12050.24177755662209666No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA11780.23636013419156007No Hit
AGCATCCTGCAGCAACTGAACCCGTGTAAAG11350.2277323873577425No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA10790.21649625194625918No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT10770.21609496139584905No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10700.21469044446941363No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG10410.2088717314884669No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT10380.20826979566285175No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT10380.20826979566285175No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10230.20526011653477583No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10230.20526011653477583No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9480.19021172089439636No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9150.1835904268126294No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC8980.1801794571341434No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8890.17837364965729788No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC8540.1713510650251208No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8400.16854203117224995No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG8290.16633493314499428No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8120.16292396346650828No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7930.15911170323761217No Hit
GTCGTGCAGCCGTCTATCCTGCAGCAGCTGA7860.15770718631117675No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT7750.1555000882839211No Hit
GAGCAGATCCGTCACGGTGATAACGGCGGTG7550.1514871827798199No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC7360.14767492255092377No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7320.14687234145010353No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG7290.14627040562448834No Hit
GGTCGTAAAGTACGCGGTGTCATTCCGACTG6870.13784330406587586No Hit
TCTATCCTGCAGCAGCTGAACCCGTGCAAAG6860.1376426587906708No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6740.1352349154882101No Hit
ATTCCGGTAGTCCAGCCGTCTATCCTGCAGC6740.1352349154882101No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6700.13443233438738986No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6640.1332284627361595No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6550.13142265525931396No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT6290.1262058781039824No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT6230.12500200645275206No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6180.12399878007672675No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6090.12219297259988121No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG6010.12058781039824074No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5990.12018651984783062No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA5970.11978522929742051No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5880.11797942182057497No Hit
CAACAGTACCAGCAGGTGCTGCAGCAGCAGA5850.11737748599495978No Hit
TCCGTCAACGGCCAGGCCAAATTCGGCGGCA5710.11456845214208897No Hit
GCGTCTGAGCTGGCCAAAGAAGGTAAAACCA5690.11416716159167883No Hit
CCAGTTGTGCAGCCGTCCATCCTGCAGCAAC5620.11276264466524343No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5580.11196006356442317No Hit
GCCCCGTTCGACACCTTCACCGGTGACTACG5450.10935167498675742No Hit
AAATCTGGCATGGAATCCCTGAAAAACATTC5430.10895038443634729No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5390.10814780333552705No Hit
ATGCTGGTGCAAGTACAGATCCCGTTCGTTC5360.10754586750991188No Hit
GGCGGTACCTACCGTAGCTATGAACAGCTGA5330.1069439316842967No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5320.10674328640909163No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5280.1059407053082714No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5160.10353296200581068No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5150.10333231673060563No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC5140.10313167145540056No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5060.10152650925376008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)