FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414375
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA41931.0118853695324284No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA38040.9180090497737556No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT31260.7543891402714932No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC26370.6363800904977376No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT19250.4645550527903469No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC18960.45755656108597287No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT17630.42546003016591255No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC14990.36174962292609353No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG13760.33206636500754144No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC13020.3142081447963801No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12960.3127601809954751No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG10780.2601508295625943No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA10610.2560482654600302No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG9890.23867269984917042No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG9440.22781297134238312No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8560.2065761689291101No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG8380.20223227752639517No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC7910.19088989441930618No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT7440.1795475113122172No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6950.16772247360482656No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG6470.15613876319758674No Hit
GGATGCGGACGCAGTGGTAGCACGAGACTGACGCGGGGCGGTAG6330.15276018099547511No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6250.1508295625942685No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6230.15034690799396683No Hit
GGACTGGTCCGGCACGTGCAGAACCGGCAGGTGCATGCGAGGCG5950.1435897435897436No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT5660.13659125188536955No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT5580.1346606334841629No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT5570.13441930618401207No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG5040.12162895927601809No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4840.1168024132730015No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4820.11631975867269985No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA4690.11318250377073907No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG4460.10763197586726998No Hit
CGGTTTGTCAACCGCGGTGGCGTCATCGTCGCTGCAATAGCTTG4380.10570135746606334No Hit
GTTGTAGAAAACGGTAGGGCTACGCGGTTCCGGGGAATCTGGGT4300.10377073906485672No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA4250.10256410256410256No Hit
TGCTGGTTTCGCGGCCGGTTTTGCGGTCGGTTTCGCAGCCGGTT4210.10159879336349925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)