FastQCFastQC Report
Wed 31 May 2023
EGAF00007935784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414375
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT93462.2554449472096527No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA63291.5273604826546003No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA28700.6926093514328808No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA24020.5796681749622926No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19060.4599698340874811No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18970.4577978883861237No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17690.4269079939668175No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC14880.3590950226244344No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13800.3330316742081448No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC11270.2719758672699849No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC11040.26642533936651586No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC10870.2623227752639517No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT9790.23625942684766216No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8880.21429864253393666No Hit
GGCCTGACTTCTTATGATGAACCGCTGGTTA7830.18895927601809956No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7240.17472096530920062No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7090.17110105580693816No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6710.16193061840120662No Hit
TCTAACAGCGTAAACGGTTCTTCCCAGGCCG6530.15758672699849172No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG6280.15155354449472097No Hit
CGTGAAACCCTGGCTAAACTGGGCGTGTCTG5820.1404524886877828No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC5810.14021116138763198No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5770.13924585218702867No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA5590.13490196078431374No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG5470.13200603318250378No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC5420.1307993966817496No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5070.12235294117647058No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG4820.11631975867269985No Hit
ACCACCCTGCCGCCGACTCCGCTGCTGACCG4760.11487179487179487No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG4530.1093212669683258No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4500.1085972850678733No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4460.10763197586726998No Hit
AAATCCCCGTCTGCAGAAGCGTGCGGCTACT4260.1028054298642534No Hit
CGTAAAGCTAAACCGGCAACTAAGCCGGCAG4260.1028054298642534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)