FastQCFastQC Report
Sun 21 May 2023
EGAF00007935793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434593
Sequences flagged as poor quality0
Sequence length44
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT47491.092746546769046No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC43591.0030074115321692No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT39160.9010729579169476No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA35140.8085726185189361No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC31800.7317191027006877No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA29570.6804067253729351No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA26140.6014823064338358No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT23420.5388950121147832No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC20790.47837862091658173No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT18190.41855253075866383No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17620.40543681099327417No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC16990.39094048914731716No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA16510.3798956725027785No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG14880.34238931598069916No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT13800.3175384785304871No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12270.2823331254760201No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA12170.2800321220084079No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10960.2521899800502999No Hit
GCGCAGCGGTGCGTCCTTATCACCGGTCGGCGCCGGAATGTCGG9010.20732041243186153No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8530.19627559578732287No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8530.19627559578732287No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8480.19512509405351675No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG8160.18776188295715762No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7930.1824695749816495No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7920.1822394746348883No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7710.1774073673529026No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC7610.17510636388529038No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7330.16866355417597614No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG6650.153016730596213No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG6390.14703412158042123No Hit
GTCTTCCGGGCCTTCCTCACCCGGGCGACGGTGCGCGCGGTGAC6290.144733118112809No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT5980.1376000073632111No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5810.1336883014682703No Hit
GTGACGACGGCGGCGTTCACCGCCGTCCGGACCTTCGCCTTCAC5760.1325377997344642No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC5740.13207759904094177No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG5720.1316173983474193No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5710.1313872980006581No Hit
ACGAACGATTACTTTCTGGCGGTCCACGATGTACAGGCCTTTGC5600.12885619418628463No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5590.12862609383952342No Hit
GTTGCACGCAGCGTTCCAGGTCTTTTCGAACGCATCAGTGCTGT5450.1254046889848663No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5140.11827157823526839No Hit
ACCCTCACCGGAACGAGCCGGACGAGAACCTTCACGGTGACGGC5130.11804147788850718No Hit
TTTCACGACCACTTTAACGGTTTCGTCACCAACAGTTACCAGTA5120.11781137754174596No Hit
GTCTGGGCCATCTTCACCCGGGCGACGATGTACACGGTGACGGC4860.11182876852595418No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA4820.11090836713890928No Hit
CGGGCCTTCACCTGGATCGTCCGCCGGACCCTGTTCGATAGCAC4460.10262475465550526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)