FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434593
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA76411.7581967496025017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55871.2855706373549505No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC55801.2839599349276218No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT46641.073188017294342No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA34890.8028201098499055No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA34890.8028201098499055No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT34480.7933859956326955No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG32190.7406930162243754No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA25880.595499697418044No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA24030.5529311332672179No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC19600.45099667965199625No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18090.41625152729105164No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG18060.415561226250768No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13490.3104053677808893No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA13330.3067237622327097No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC12600.28992643691914044No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11750.2703679074444365No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9540.21951573081020634No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9290.2137632221411758No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC9210.211922419367086No Hit
GTTGTGAACAAAATTGATCTGCCGTCTGCTG9020.20755051277862274No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8830.2031786061901595No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG8810.20271840549663708No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8530.19627559578732287No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8470.19489499370675553No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT7990.18385017706221682No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7960.18315987602193318No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT7960.18315987602193318No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7910.18200937428812705No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC7690.17694716665938015No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6810.15669833614439257No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6410.14749432227394368No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC6360.14634382054013753No Hit
GGCGAAGCTGAACGTGGCAAAGCCGGTGATC6030.13875050909701722No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6030.13875050909701722No Hit
ACCAAACAGTATCATCGCTCCTCTCGTAACC5760.1325377997344642No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5750.13230769938770298No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5690.13092709730713564No Hit
ACCGCCACCGTTAAAGCATATGGCGGCGAAG5680.1306969969603744No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5630.1295464952265683No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5540.1274755921057173No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA5290.12172308343668674No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5030.11574047442089495No Hit
GCTGAACGTGGTAAAGCAGGTGACCCGCACC5000.11505017338061128No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG4920.1132093706065215No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG4490.10331505569578893No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4360.10032375118789305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)