FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383454
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56161.464582453175609No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT47151.2296129392313029No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC27340.7129929535224564No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA23730.6188486754604203No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG22070.575557954800315No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG21100.5502615698362776No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20290.5291377844539371No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG20060.5231396725552478No Hit
ATGTCCAACATGGACATCGATGGTATTAACA18570.4842823389506955No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG17130.4467289427154235No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG16460.4292561819670678No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG15280.39848326005205315No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA14860.3875301861500988No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC14510.3784026245651369No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG14390.37527317487886425No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC13440.35049836486253894No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC12510.3262451297939257No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG11760.3066860692547216No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC11000.2868662212416613No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC10970.2860838588200932No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG10150.2646992859638966No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA10000.2607874738560557No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC8370.21827911561751864No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8060.21019470392798092No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8010.20889076655870065No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA7790.20315344213386743No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7690.20054556739530688No Hit
GCTCTGACCCTGAGCCTGCCGAAACAGCAGG7250.1890709185456404No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC7050.1838551690685193No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6920.18046493190839058No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG6400.1669039832678757No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG6390.16664319579401962No Hit
GCACTGACCCTGAGCCTGCCGAAACAGCAGG6260.16325295863389092No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG6230.16247059621232274No Hit
GCGCTGACTCTGTCCCTGCCGAAACAGCAGG6100.159080359052194No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6060.15803720915676978No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG6030.15725484673520163No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC5800.15125673483651234No Hit
GACCCATTCCTGGACGGCTCTCACTCTGTTA5670.1478664976763836No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5490.14317232314697462No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5400.14082523588227008No Hit
CCGCAGCAGCAGCAGCGCCACGCGGCTTTCT5290.1379565736698535No Hit
GCCAACTACATCGTCCGTCAGAGCCGCGGTG5270.13743499872214138No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA4970.1296113745064597No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA4800.12517798745090675No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG4740.12361326260777042No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA4720.12309168766005832No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG4670.12178775029077803No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG4650.12126617534306593No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT4630.12074460039535381No Hit
TCCTACACCAACGGCAAACTGACTATCACCC4570.11917987555221747No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG3980.1037934145947102No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG3930.1024894772254299No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG3880.10118553985614964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)