FastQCFastQC Report
Fri 21 Apr 2023
EGAF00007935816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418397
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC55921.3365296596294907No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA45841.0956101501683806No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG37560.897711981682469No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT35100.8389161490163648No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA23770.5681207083224784No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23180.5540192687806079No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT18500.44216378224509256No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG18380.439295692846746No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16890.4036835828172764No Hit
GCTGAACGTGGTAAAGCAGGTGACCCGCACC16860.4029665604676898No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT15420.3685494876875312No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT14280.341302638403239No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14100.3370005043057192No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13960.33365440000764823No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT13900.33222035530847494No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT13830.3305473031594395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13710.32767921376109294No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC13590.3248111243627464No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA13160.31453380401867126No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG13050.31190472207018693No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA12730.3042564836745961No Hit
GGCGAAGCTGAACGTGGCAAAGCCGGTGATC12150.29039405158258785No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG12120.2896770292330012No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT11230.2684053661952643No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10380.24808973295697626No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC10200.24378759885945647No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC10140.24235355416028317No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10040.23996347966166107No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9960.23805142006276336No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA9100.21749677937461312No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA8970.21438968252640433No Hit
TCTGCCTGCAACGATTTCAGCGTTCGTATGC8680.20745846648040017No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8520.20363434728260482No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8340.19933221318508498No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT8310.19861519083549833No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG8230.19670313123660066No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8180.19550809398728958No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8030.1919229822393564No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC8000.19120595988976977No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC7970.19048893754018312No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG7800.18642581089252552No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC7690.18379672894404117No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7610.18188466934514347No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG7570.18092863954569466No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC7350.17567047564872598No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7260.17351940859996606No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG7190.17184635645093058No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT6950.16611017765423747No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC6750.16133002865699322No Hit
AACACTTCTAGCGGTCAGACCCAATCTACCA6500.15535484241043793No Hit
GCTTCCGAACCGGAGGACAAATCTCCGCGCG6280.15009667851346925No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT6190.14794561146470936No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6180.14770660401484714No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC6130.1465115667655361No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5900.1410143954187052No Hit
CAGAACCTGACCCTGCGTATCCAGGTTTCTA5550.1326491346735278No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5530.13217111977380336No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5380.12858600802587017No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5230.12500089627793698No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG5120.12237181432945266No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC5030.12022074728069274No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5010.1197427323809683No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT4860.11615762063303514No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA4810.11496258338372406No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4640.11089945673606647No Hit
CCGCTGCCGCCGGCAACGCTGACCGTTCCGC4630.11066044928620426No Hit
GCCGATGGCGAACCGCGTCGCCACCGTGTCC4380.10468526303964894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)