FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007935817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414990
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT21550.5192896214366611No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA19300.46507144750475915No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA19190.46242078122364394No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC18700.45061326778958527No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA16660.40145545675799416No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT13540.3262729222390901No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12690.30579050097592714No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12180.29350104821802936No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG10220.24627099448179474No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT9070.21855948336104483No Hit
GCGAATGTGACGAGTCAGTTCATCAGAACGGGAGAAGCGACGGT8920.21494493843225138No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT8840.21301718113689488No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC8280.19952288006939928No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT8270.19928191040747967No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8240.198559001421721No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT8050.19398057784524927No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7990.1925347598737319No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7980.19229379021181234No Hit
ATGACGGGTCAGTTCGTCAGAACGGGAGAAACGACGATCGCAAC6790.16361840044338416No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG6550.15783512855731463No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT6410.15446155329044073No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6050.14578664546133643No Hit
TCAGCATTTACCGAAGTGAGACAGGGTCAGGGTGCCGTTAGATT5940.1431359791802212No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5500.13253331405576038No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT5400.13012361743656475No Hit
AGGGTAAACGTCCGGCATTTCGTTTGCCGGTTTTACACGTGGCT5370.12940070845080606No Hit
ACCTGCACCAACTACGACCGGTTCCTTCAGCGGAGTGTGCAGTT5350.12891876912696693No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5130.1236174365647365No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5130.1236174365647365No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5020.12096677028362128No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG4930.11879804332634522No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC4870.11735222535482782No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC4680.1127738017783561No Hit
GCATTTACCGAAGTGGGACAGGGTCAGAGTACCGTTGCTTTCCA4460.10747246921612569No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT4450.10723149955420613No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG4430.106749560230367No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4350.10482180293501049No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT4350.10482180293501049No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG4180.1007253186823779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)