FastQCFastQC Report
Wed 31 May 2023
EGAF00007935818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414990
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63241.5238921419793248No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT46171.1125569290826285No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC25110.605074821080026No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21220.5113376225933155No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA18120.43663702739825055No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17200.4144678185016506No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA14810.35687606930287474No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14480.34892407045952917No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13420.32338128629605534No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10890.26241596183040555No Hit
CTGAACACCTCTTACCAGAGCCAGCTGATCA9310.2243427552471144No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG9100.21928239234680355No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8970.2161497867418492No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG8860.213499120460734No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8840.21301718113689488No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA8500.2048242126316297No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8220.1980770620978819No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA7550.18193209474927108No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA7540.1816911250873515No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC7390.17807658015855804No Hit
GCTGCCGCGTACAACCCGCACCACCTGCCTC6930.1669919757102581No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6500.1566302802477168No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6350.15301573531892335No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA6310.15205185667124507No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5820.14024434323718643No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5680.13687076797031253No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5630.1356659196607147No Hit
GTGAGCGTAGACTGCTCCGAATACCCAAAAC5610.1351839803368756No Hit
ATGTCTCTGGCAACTGATATCCTGAAACACG5500.13253331405576038No Hit
GTGCAGGACGCCACCGTTGGCGCAGAAAAAG5300.1277139208173691No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5180.12482228487433432No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5170.12458131521241476No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4690.11301477144027568No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC4610.11108701414491914No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4400.1060266512446083No Hit
GCAGTTTCTGTTGACTGCTCTGAATACCCGA4250.10241210631581485No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4240.10217113665389528No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT4240.10217113665389528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)