FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449832
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT20560.45705952444468156No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC17810.3959255899980437No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG14420.32056412171655196No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG14330.31856337477102564No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA13560.30144587312596705No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12630.2807714880221949No Hit
ACGAGAAGCCGCGGAGGTCGGAGCTACGGCCGGATCCGGTTTGC12090.268767006349037No Hit
CCAGGCAGAACCGTGGTGGTTACGGGATGCGCTACCAGAATGGC11730.26076401856693165No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG11240.24987106297462164No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC10820.24053424389549877No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC9160.20363157801134646No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC8770.19496167458073235No Hit
GCTGGCAGCGGAGGTAGGTGCTACCGCCGGGTCCGGCTTACGGG8710.19362784328371482No Hit
AGACGCTGCGGAGGTCGGAGCAACAGCCGGATCCGGTTTACGGG8420.18718099201479663No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC8290.18429102420459195No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG8000.17784417293567378No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA7060.1569474826157321No Hit
CTTGCGAGACGCTGCGCTAGTAGGGGCAACCGCCGGGTCCGGTT7000.15561365131871455No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6340.14094150705152145No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6340.14094150705152145No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA6250.13894076010599513No Hit
GAAGATGCTCGGACCGATACCAGTCCAAGACGGACGGTACTGAA6190.13760692880897757No Hit
TCATTTGAAACGCTGCATCTGGACGCCCATACGTTTCTGGTAGG6130.13627309751196No Hit
CTGCATCTGCACACCCATACGTTTCTGGTAGGCTTGCAGGTTCT5820.12938163581070267No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG5740.12760319408134593No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC5590.12426861583880204No Hit
TGGGTCCGGCTTGCGGGTAGCTGCGCTAGTCGGGGCGACAGCCG5550.12337939497412367No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5420.12048942716391899No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA5330.11848868021839265No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG5180.11515410197584877No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5080.11293104981415283No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG5030.11181952373330488No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT4780.10626189332906506No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG4750.10559497768055631No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG4580.10181578900567323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)