FastQCFastQC Report
Wed 31 May 2023
EGAF00007935826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449832
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66071.4687705632324957No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA42210.9383503174518486No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30330.6742517206423732No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA17930.3985932525920788No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG15260.3392377598747977No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG14080.31300574436678585No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA13410.29811129488342314No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG13140.2921090540468442No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG12280.27299080545625926No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC11670.2594301872699141No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11340.2520941151363176No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG10480.23297586654573263No Hit
CCGGACCCAGCGGTGGCACCGACTTCTGCGG10140.2254174891959665No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG8330.1851802450692703No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7610.16917426950505968No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT7230.16072667129061516No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6570.1460545270234221No Hit
CCGGCCGTGGCACCAACCAGCGCTGCCTCCC6500.14449839051023494No Hit
AGCGAACAGGCAGCGGAAGCGATGGAAGTTG6380.14183072791619983No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6160.13694001316046878No Hit
TCTCAGAACTCCCGTGCCTCCATTGGCTCTA6100.13560618186345125No Hit
GCAGAAGCGATGGAAGTGGCGAACAAAACCC5990.13316082448558572No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG5950.13227160362090734No Hit
GCACCGACCTCCGCGGCGAGCCGTAAACCAG5760.1280478045136851No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG5740.12760319408134593No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5730.12738088886517632No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5680.12626936278432838No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5470.12160095324476694No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5220.11604332284052712No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5190.11537640719201836No Hit
ATGTCCAACATGGACATCGATGGTATTAACA5110.11359796546266163No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG4880.108484945490761No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4740.10537267246438671No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4660.10359423073502998No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4620.1027050098703516No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC4580.10181578900567323No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT4520.10048195770865569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)