FastQCFastQC Report
Sun 21 May 2023
EGAF00007935860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539562
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA57211.0603044691805574No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42970.7963866988409116No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT29630.5491491246603727No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA26040.48261367553682427No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA25860.479277636305003No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA24020.44517590193527345No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18360.3402760016457793No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG16360.3032088990699864No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16110.2985755112480123No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG14550.2696631712388938No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC9920.18385282877593306No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9320.17273269800319518No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9180.17013800082288966No Hit
GACGCTAACGGCAACAACCTGACTTTCGAAC6550.12139476093572193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)