FastQCFastQC Report
Wed 31 May 2023
EGAF00007935863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489086
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT47680.9748796735134516No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC45210.9243773078763244No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC38230.781662120772216No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG25060.5123843250471287No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC24080.4923469492072969No Hit
GTACATGTGCGGACGGTGCGGATGGTTATCGAAACCGTGCGGGT20990.4291678764061944No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG20550.42017150358014743No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20440.4179224103736357No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG18720.3827547711445431No Hit
CGCATCAATAACAGCTACAACGGTACCAGCGCCTTTACCTGCCT15220.31119271457371506No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT12960.2649840723308375No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG12230.2500582719603505No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG11840.24208421422817253No Hit
GCTCTGGTAGCGTGCTTTGGTCTTATCAGTCAGACGCCAGGCTT11800.24126636215307737No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG10920.22327361650098349No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT10400.21264153952474615No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG9890.20221392556728263No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG9650.19730681311671158No Hit
TGGACGACGCGGGCGCTGCGGTTTGGTCGGGGTCGGTTTCACAG9240.188923829346986No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG9110.1862658101029267No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8750.1789051414270701No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT8700.17788282633320113No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG8670.17726943727687974No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA8480.17338463992017764No Hit
CAGCTCGTCTTCGTCGCCCGCTTCATCTTCGTTGTGCAGCGGGG8070.16500165615045206No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG7880.16111685879374998No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7640.1562097463431789No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG7400.15130263389260784No Hit
ACGAATAGTGATGTTGTCATACTTCAGCAGCTCATCCGGGTTGT7390.15109817087383406No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG7250.14823568861100095No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG6890.14087501993514434No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6840.13985270484127535No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG6610.1351500554094781No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6520.13330988824051393No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6430.13146972107154978No Hit
GCCTGCGGTCTGGCCGTTCGCGGCTTCATAAGACCAGGACCAGG6230.1273804606960739No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG6130.12533583050833597No Hit
TGGATCGTTCAGGTCACGGATAGTGGCCTTGGACGTAGCCGGAG5670.1159305316447414No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5520.11286358636313451No Hit
ACCGTCTTTGTTCGGCACCTGGGTGTAGTTGGTGTTACGACGTT5230.10693415881869446No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG5090.10407167655586133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)