FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489086
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA99632.0370650560433132No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA53161.086925407801491No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA49581.0137276470804726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42830.8757151094081613No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG22740.46494890469160843No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC22500.46004179224103736No Hit
GAATGCGACAACTATCGCCAGGAATACGATA20640.4220116707491116No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20310.41526439112957636No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG19440.39747610849625625No Hit
ACTCCGTCTGACGCAGGCGAAACGGTCGCGG18910.38663956850124515No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC13380.2735715191193369No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA13290.27173135195037273No Hit
GCTAAAACCGCAGATACCCCGGCGACCTCTA12770.26109927497413543No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT11610.2373815647963753No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA11560.2363592497025063No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG9570.1956711089665212No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG9490.19403540481633086No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG9480.19383094179755708No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9250.18912829236575981No Hit
ATCGAAGGTACTGACGATAAATGCGAGGAAG8960.18319886482131975No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8630.17645158520178456No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC8580.1754292701079156No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8010.1637748780378093No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA7920.16193471086884517No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7710.15764098747459546No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7330.1498713927611913No Hit
ATGCAGCAGCAGGCATACATTAACGCGACCA7270.14864461464854853No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG7220.14762229955467956No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7140.1459865954044892No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6970.1425107240853347No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG6380.1304474059776808No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6260.12799384975239528No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6150.12574475654588355No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6100.12472244145201458No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC5970.12206442220795526No Hit
GTTGAAACCCCGCAGCCGACCGCTGTATCCG5730.11715730975738417No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5530.11306804938190829No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT5220.10672969579992068No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC5140.10509399164973031No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT4990.1020270463681234No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG4970.10161812033057582No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC4940.10100473127425444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)