FastQCFastQC Report
Sun 21 May 2023
EGAF00007935874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563524
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT33940.6022813580255677No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT13290.23583733789510294No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA10690.18969910775761104No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA10240.18171364484919897No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9650.17124381570261427No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9190.16308089806290416No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC8480.1504816121407429No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8340.14799724590257027No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA7870.13965687353156211No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA7870.13965687353156211No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7810.13859214514377383No Hit
CTGTCCTCCATGGCTATCACCTTCAAACGCG7580.13451068632391877No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7160.12705758760940083No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7030.12475067610252626No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7010.12439576663993016No Hit
ACCTACCTGACGCAGGTACCGCCGGAACGTG6740.11960448889488291No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC5840.1036335630780588No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG5700.10114919683988616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)