FastQCFastQC Report
Wed 31 May 2023
EGAF00007935890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585015
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTTTCCTGGGCGATCTGGTTGACCGTG161002.7520661863371028No Hit
TACAGGAAGGTGTCCTGACCTGGTACGAAGA62411.0668102527285626No Hit
AAGATTCTGCTGCAGCGTTACGGTGATTTCC49970.8541661324923292No Hit
GCGCTGACGGCCACCTTCTCCAACATCGTGG47350.8093809560438622No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47320.8088681486799483No Hit
ATGCACGTGATTTCTCGTAAACCGTTCAACG42530.7269899062417203No Hit
CGTACCGTAAAAGGTGGCACCATCGAGCCGC36890.6305821218259361No Hit
ATGCTGTGGGCTCGTCAGGTCATTTTCATGA35440.6057964325701051No Hit
GTTGGCGGCGTAGGCGCAGGCGCAGACCCGT28100.4803295641992085No Hit
TTCTCCAGTCAGTCTGGTAGCTGGTACCTGA27630.47229558216455986No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA24800.4239207541686965No Hit
TCTACTCTGATCCGCCTGATGTCGGTGTTGA17790.3040947668008513No Hit
ATGGACATCTGTCTGCGTTTCTTCTGCGCGG15100.2581130398365854No Hit
CAGTCCTTTACCGAATGGCAGAACCCGGAGG14680.2509337367417929No Hit
GACCAGAGCACTCTGGGCTCTAATTGGGGTG14070.24050665367554677No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11450.19572147722707964No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA11300.19315744040751093No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA9840.16820081536370862No Hit
CCGGCGTCTAGCGGTTTCGCAAGCGTTATGC8460.14461167662367633No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG8370.14307325453193506No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG8370.14307325453193506No Hit
GACAGCATGCGTACCATCGATCAGACCTACA7740.1323042998897464No Hit
ACTATTCTGACCTACGCACCGAAAGCAATTG7170.12256095997538524No Hit
GGTTCTTCCAAAGGTGACGTTGCAGAGGGTG7120.12170628103552901No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6880.11760382212421902No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG6880.11760382212421902No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6220.10632206011811664No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6190.10580925275420287No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6010.10273240857072041No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5950.10170679384289291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)