FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457523
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA50551.104862487787499No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA45090.9855242250116388No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT42400.9267293666110775No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC38920.8506676167099795No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC35080.7667374099225612No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG33680.7361378553646484No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT28950.6327550746082711No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC22260.4865329174708157No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG20580.44981345200132017No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA18330.40063559646181723No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT15780.3449006935170472No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG15160.3313494622128287No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT14170.30971120577544736No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG13580.29681567921175545No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG12990.2839201526480636No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA12650.2764888322554276No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC12290.2686203753691071No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG12220.2670903976412115No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT10750.2349608653554029No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9720.21244833593065268No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9650.21091835820275703No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG9220.2015199235885409No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG9080.19845996813274963No Hit
AGCGCCGGTCGGAGGCGGGCAAACGTAGAAGTTTGCGTCGGTGT8970.19605571741748504No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG8920.19496287618327385No Hit
CACGCAGGCACCACGACGGCGACGAGACGGCGGCGGAGTAGGTT8390.1833787591006354No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT8360.18272305436010866No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC7480.163489048637992No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC7410.16195907091009631No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT7230.1580248424669361No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA7160.15649486473904042No Hit
AGACAGGCTTTTACGAGTGGACGCAACCTGTTTACCGACAGCGT7010.1532163410364069No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG6650.14534788415008645No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG6500.1420693604474529No Hit
CGCCTGCGGGCACGGACGACCGTGAATGGACAGATCCAGTGCTT6360.13900940499166162No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG6250.13660515427639702No Hit
TACGCAAGCGCCACGGCGACGACGGCTCGGCGGCGGGGTCGGTT6080.13288949408007902No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC5990.13092237985849892No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA5520.12064967225691386No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5440.11890112628217599No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5410.11824542154164927No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT5230.11431119309848903No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5200.11365548835796233No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG5200.11365548835796233No Hit
GGAAGGCGGCGGGGTCGGTTTCACCGGCACCGGTTTCGGAACTG5090.11125123764269775No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA4950.10819128218690645No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4930.10775414569322198No Hit
CGGTTTATCTTTCTCGACGTCTTCCGCCAGCGGGAATGCCGGGC4930.10775414569322198No Hit
ACCCGGAGACTGCGGCAGCGTATCACGGGTGCCGTCGCCACCCT4760.10403848549690399No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG4670.10207137127532387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)