FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457523
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT114192.4958308106914844No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA87561.913783569350612No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA47631.0410405597095667No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA46041.0062882084616511No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG34760.7597432260236098No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC27960.6111168181708898No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23150.5059854914397746No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC19530.4268637860828855No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA16240.3549548328717901No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC15920.3479606489728385No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC14380.3143011389591343No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14020.3064326820728138No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA13560.296378542718071No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12590.2751774227743742No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC11930.26075191848278667No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10380.2268738402222402No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC10270.22446958950697563No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC10030.21922395158276198No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA9960.21769397385486633No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9860.21550829138644395No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC9380.20501701553801666No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT9120.19933424112011855No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG8970.19605571741748504No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8530.18643871455642666No Hit
GACAAAGCGGAAGCTCAGAGCACCCCGGAAC8360.18272305436010866No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA8140.1779145529295795No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC7880.17223177851168137No Hit
GACAAAGCGGAGGCCCAGTCTACCCCGGAAC7860.1717946420179969No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA7690.1680789818216789No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG7400.1617405026632541No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA6780.1481892713590355No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC6690.1462221571374554No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA6640.1451293159032442No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG6510.14228792869429516No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC6040.13201522109271008No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5930.1296109703774455No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5840.1276438561558654No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5620.12283535472533622No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5430.11868255803533376No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC5420.1184639897884915No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5400.11802685329480704No Hit
GCTGAAGCGCAGTCTACCCCGGAGCGTCCGG5370.11737114855428032No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG5280.1154040343327002No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA5200.11365548835796233No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4980.10884698692743316No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT4950.10819128218690645No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG4800.10491275848427294No Hit
GAAGCCGAGCTGGTTAACGACACCACCGCTC4770.10425705374374622No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT4760.10403848549690399No Hit
CACAAAGCAAAACACATCAAGGCCTGGTGCC4660.10185280302848163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)