FastQCFastQC Report
Wed 31 May 2023
EGAF00007935954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522499
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT75401.4430649628037564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47920.9171309418774007No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC45210.86526481390395No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA27390.5242115295914441No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG23090.44191472136788773No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT21720.4156945754920105No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG16530.31636424184543893No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16460.3150245263627299No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC16060.3073690093186781No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13870.26545505350249476No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA13710.26239284668487406No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC11650.22296693390800748No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG11590.22181860635139972No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC10430.1996176069236496No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT10390.19885205521924443No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10370.19846927936704187No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC10110.1934931932884082No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9660.18488073661385No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8670.16593333192982188No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8460.1619141854816947No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG7610.1456462117630847No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7100.13588542753191873No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC7070.13531126375361482No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT6900.13205766900989285No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6880.13167489315769026No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC6210.11885190210890355No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6020.11521553151297896No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5890.11272748847366215No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5860.11215332469535827No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5680.10870834202553498No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5660.10832556617333239No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5570.10660307483842074No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA5500.1052633593557117No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC5370.10277531631639486No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC5280.10105282498148321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)