FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517358
Sequences flagged as poor quality0
Sequence length31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC63891.2349282315147345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46760.9038228847335887No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC45570.8808214041340813No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG39670.766780449901229No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA37290.7207774887022139No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC34960.6757409762678842No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC32630.6307044638335544No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT30330.5862478206580356No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG28100.5431442057530762No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG24520.4739464742016167No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC24210.46795449186056853No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC23940.4627356685312685No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22820.4410872162023203No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19920.38503318785057933No Hit
GGCGAGTTCCGCCTGCACAACCCGATCAAAG19590.37865462600365707No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC16230.31370926901681234No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC16040.31003676371100863No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT15590.3013387248288419No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC15420.2980527990289123No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG15220.2941870039701715No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC14560.28142988027632704No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG14420.2787238237352085No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG14330.27698421595877515No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT14040.27137881312360107No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12340.23851955512430464No Hit
CTGAAGGCGACCACCACCCACCTGTTCGAAG11890.22982151624213795No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA11640.22498927241871197No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC11140.21532478477186012No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC10430.20160121231333042No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA10370.2004414737957082No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC10010.19348304268997485No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT9920.1917434349135415No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT8710.16835537480815993No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC8700.1681620850552229No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC8690.16796879530228584No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG8690.16796879530228584No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC7940.153472063832008No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA7800.15076600729088946No Hit
GGTTACCAGGAACCGCCGGCCCCGCAGGCAC7670.148253240502708No Hit
CCGCCTGCGCCGCAGGCGCCGTACCAGGGCT7570.14632034297333762No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7500.14496731470277835No Hit
GGTGACGCGCGTGAAAACGACGTGTTCGAAC7130.13781559384410794No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7070.13665585532648572No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6750.13047058323250052No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT5990.11578056200928565No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5950.11500740299753749No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5900.1140409542328523No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC5890.11384766447991526No Hit
TCTACCCAGCACACTGTTCAGTCCGGCGAAA5720.11056173867998562No Hit
GCCCAGTCTTCCGGCGATCGTCAGACCGTCT5510.10650265386830783No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG5470.10572949485655968No Hit
TCTCCGACCGAAAAAGCGGGTGCATCCGGCC5440.10514962559774856No Hit
CCACCGGCCCCGCAGGCTCCGTACCAGGGCT5440.10514962559774856No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT5420.10476304609187449No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG5300.10244356905663003No Hit
GTCCCGGATACTCCGCTGTATGCAGCCAAAA5280.10205698955075597No Hit
CGCCCGCCGGAGCTGCAGGGTGAACGTTGCG5280.10205698955075597No Hit
GTGGATATTAAAACCAAAGAAGCGTACGTTA5230.10109054078607077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)