FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007935987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007935987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538857
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC48500.9000532608836853No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG45700.8480914231419467No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT41520.7705198225132085No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA37500.6959174697554267No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG35430.6575028254249272No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG32840.609438125513819No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA29620.5496820121108198No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA26700.49549323846586385No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT24930.46264593389340775No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA24240.44984105244990785No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT21440.39787921470816934No Hit
GTGCTGGCTACGCGGGGTCCACGGACCAGCATAGCCCGGGTAGG21320.39565227880495196No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT20030.37171271784536525No Hit
GGACTGGTCCGGCAGGGTATCCGGGAAAGCCGCACGTGCCGCAC20010.37134156186149575No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG16450.3052757967327139No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT15900.2950690071763009No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT15680.29098629135373577No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC13980.2594380327248231No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13850.25702551882967095No Hit
CGGGTCCTGGGACCACGGAGCCCACGGATGACGGTAGCACGGGT13790.2559120508780623No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC12540.23271480188621468No Hit
GGCAATGCCGGCGCGCCAACGACCATCACGCGCTTTGTCAGTGA12480.23160133393460602No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG12470.23141575594267122No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11470.21285795674919322No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG10580.19634151546699774No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA10220.18966070775734564No Hit
CGGAGCCTGCGGTGGCGGCGGCTCTTGATAACCCTGATACGGCG9850.18279432205575877No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9450.17537120237836754No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG9370.1738865784428893No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC9070.16831923868484588No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC8720.16182400896712856No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC8600.15959707306391122No Hit
CGGTGCCTGTGGCGGAGGCGGTTCCTGATAGCCCTGGTATGGTG8580.15922591708004166No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC8490.15755571515262862No Hit
GTGCTGGGAACGCGGGGTCCAAGGGCCAGCGTAGCCCGGATAGC8380.15551435724134602No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC8180.15180279740265043No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7750.14382294374945487No Hit
TGGGTCTTGAGACCACGGTGCCCAAGGGTGGCGATAGCACGGGT7570.1404825398946288No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT7280.1351007781285202No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7130.13231710824949847No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6990.12971901636241154No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG6880.12767765845112897No Hit
GTTCAGGCCGCGAATACCGAAGTTCATAGCCAGGTCGTGAGAAC6630.12303820865275944No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA6560.12173916270921599No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG6370.11821318086245515No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA6360.11802760287052039No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6280.11654297893504213No Hit
CGGGATAGTGCCGTTGTCGCCGCCGGTCAGTTTGGTCAGCGGAA6210.11524393299149867No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6130.11375930905602041No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6110.11338815307215086No Hit
CTCTGGATTTAAATATGTCTATCAGGTGGACGGGCCTTCACCTG6110.11338815307215086No Hit
ATAAATACGGCTGCAGTCGATGTCCGGGCTAATGGTGTACGGCA5920.10986217122539003No Hit
CGGGCAGGACAGACCAGAGGTCAGCTTACAGCGGCATACGTTTG5810.10782081331410745No Hit
ATCGTCGAACGGGATATACTGCACGAAGCCGTCGTAAGCGGTAA5720.10615061138669443No Hit
CGGGTCCTGAGACCATGGCGCCCATGGGTGACGGTAGCAAGGGT5700.10577945540282488No Hit
GGACGCGCTACCTGCCTGCGGAGAGGTGTTAGAGGTGTTCTGGG5640.10466598745121618No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT5580.1035525194996075No Hit
CGGCAGTGGGTCCAGGAAGTGCAGTGGCTGCAGCTGCGGCTGTT5500.10206789556412926No Hit
TTCCTGACCTTCCTCACCTTCGTCGCCATCGCCACCTTCATCAC5480.1016967395802597No Hit
GACCGGCCATTCCGGGGTGCGCGGACGTGGACGACCGTGAATAG5460.10132558359639014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)