FastQCFastQC Report
Wed 31 May 2023
EGAF00007936062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007936062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505252
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA71601.417114627948034No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA34530.6834213422213073No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA31760.6285972148551614No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC28700.568033377403751No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC26100.5165739076737945No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG25480.504302803353574No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG24860.49203169903335364No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG22350.4423535186402033No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT17380.34398676304101716No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG16570.32795515900976147No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15510.306975529042933No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT14970.2962877930220959No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT14970.2962877930220959No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA14640.2897563987871399No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC14630.28955847774971694No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT14410.28520421492641296No Hit
CCGAAGAAAAACCGTAAACCGAAGCCACCGA13650.2701622160822718No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11850.23453642934614807No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC11270.22305700917561927No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA10600.20979629966828436No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC10040.1987127215726014No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9590.18980627488857046No Hit
CACGAAGCTCCGAACATGACCCAGACCGGTA9380.18564993310268935No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA9360.18525409102784351No Hit
GCACTGGGCCAGCGTCGTGCCGATGCAGTTA9250.18307695961619153No Hit
TCCTACACCAACGGCAAACTGACTATCACCC8910.1763476443438126No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8830.17476427604442932No Hit
GAACCGATCTACGCTACCATCGACGACCTGG8830.17476427604442932No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8770.17357674981989185No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT8720.1725871446327773No Hit
CGTGGCGAATTTGAACTGGTTGCGCAGCGCA8320.1646703031358609No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC8050.15932643512544237No Hit
GAAGTTGGCCGTAAACGTGTATTCCAGGAAA7980.15794098786348199No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7640.15121167259110305No Hit
GCGCTGGGCCAGCGTCGCGCTGACGCAGTTA7570.1498262253291427No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA7370.1458678045806845No Hit
GATTACGGTAAGAAACAGGACAAAGCCCTGG7040.13933641034572847No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT6860.1357738316721161No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6660.1318154109236579No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6590.13042996366169754No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG6550.1296382795120059No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT6400.12666946395066225No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6400.12666946395066225No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC6300.12469025357643314No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6280.12429441150158733No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG6240.12350272735189569No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG6160.12191935905251242No Hit
CACACCCCTCCGATGCCGTCTATCGGTCTGG6130.12132559594024367No Hit
GAACGTGTCGACATGCTGAAGGCGGGTGCAA6070.12013806971570623No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG5840.1155858858549793No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5820.11519004378013349No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5750.11380459651817311No Hit
GTCGGTGACGGTGAGCCAACGACCGATGCTA5640.1116274651065211No Hit
AACGGTTTTGTAGCTTGGGATTCCCAGTACA5630.11142954406909819No Hit
CGTACCGTTGAATATTCCCCATTCGCTGGTC5560.11004409680713784No Hit
GAACGTGGTAACTTCTCCAAATTCACCCTGG5460.10806488643290872No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5380.10648151813352545No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5320.10529399190898799No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5160.10212725531022143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)