FastQCFastQC Report
Wed 31 May 2023
EGAF00007936260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007936260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459749
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67291.4636247169651266No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA58801.278958736179959No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA27100.5894520705863416No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23330.5074508046782049No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21530.46829900663187957No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG21160.4602511370334682No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT20870.45394334734822694No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15850.3447533327968087No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC14870.32343735386047606No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC14460.31451944430547973No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13400.2914633854559771No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC12740.27710772617232443No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12120.26362210684525683No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC12070.2625345568995256No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG11540.2510065274747743No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10500.2283854886035641No Hit
GCCGAGGGTCTGAAGGACGCGATTAAAGACC10290.22381777883149287No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9370.20380685983003768No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT8930.19423642030760263No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC8870.19293136037272512No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC8720.18966871053553133No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC8050.1750955412627325No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7930.17248542139297748No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT7780.16922277155578372No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7680.16704767166432116No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG7340.15965233203334864No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT7270.15812976210932486No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7180.1561721722070086No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA6580.14312157285823351No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT6340.1379013331187235No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5910.12854840358543465No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG5690.12376318382421714No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC5590.12158808393275462No Hit
ATGTCTGACGAAGGCCCGGGCACCGGTCCGG5520.12006551400873085No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA5460.11876045407385334No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5450.11854294408470709No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT5160.11223515439946578No Hit
GAATGCGACAACTATCGCCAGGAATACGATA5070.11027756449714954No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC4860.10570985472507825No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4790.10418728480105449No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC4680.10179467492044572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)