FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007936435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007936435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524474
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC40030.7632408851535062No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT34880.6650472664040543No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA33300.6349218455061643No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA29860.5693323215259479No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG28950.5519816044265302No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT28190.5374908956402034No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG25610.488298752655041No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA24600.4690413633468961No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG22700.4328145913810789No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT22380.42671324031315183No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC21130.4028798377040616No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG21090.40211716882057075No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA20850.3975411555196253No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG20840.3973504882987527No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA18880.35997971300769915No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG18700.3565477030319902No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT18290.3487303469762085No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG17880.34091299092042693No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA17290.32966362488893636No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT16880.3218462688331547No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT14910.28428482632122853No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG14270.2720821241853743No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC14140.2696034503140289No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14130.2694127830931562No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG13650.26026075649126557No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG13490.25721008095730197No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA13360.2547314070859566No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT13040.24863005601802948No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT12570.23966869663701157No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA12200.23261400946472086No Hit
CTGGCCGCGGTGTTTACCGAACCAGCCGCCTTTTTTACGACGGC11940.22765666172203008No Hit
CTGGCCACGGTGTTTGCCGAACCAGCCACCTTTTTTACGACGAC11570.22060197454973934No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA11440.21812330067839397No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA11280.2150726251444304No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT11180.2131659529357032No Hit
ACCACCTTTTTTACGGCGGCCGCCATCACCCTGACCACGTGGAC10910.20801793797213972No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA10830.20649260020515792No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT9860.1879978797805039No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT9270.1767485137490133No Hit
CTGGGAGGTCAGGAACACCAGGTAGCTGCGCTGAGCTTCACGGA8770.16721515270537718No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8500.1620671377418137No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG8360.15939779664959558No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG8310.15844446054523198No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG8310.15844446054523198No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG7770.148148430618105No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7730.1473857617346141No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG7680.1464324256302505No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA7680.1464324256302505No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG7550.14395375175890512No Hit
CACATCGTCACGGAACTGTTTGGTCGGCATGGACAGGGTCAGAG7290.13899640401621435No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7090.1351830595987599No Hit
CAGTTCGGTCAGCATCGGCAGGTCAACCGCGATGCCCGCAGATT6880.13117904796043273No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6700.12774703798472373No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG6700.12774703798472373No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC6700.12774703798472373No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT6450.1229803574629057No Hit
CAGCGCGCGCAGGCCCTCAGCGATGTTTTCGAACTTCGGGTTGG6110.11649767195323314No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT6030.11497233418625136No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG5600.10677364368872433No Hit
CAGTGCACGCAGGCCTTCAGCGATGTTTTCGAACTTTGGGTTGG5530.10543897314261527No Hit
AGATTCAACATCCGTTTCGCTACCGTTGAAGTGCATGTAGGTGG5520.10524830592174256No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT5500.1048669714799971No Hit
TTCCTGGTGATACTCAAAGTAATCAGCTTCACCCACCGGATGGA5480.10448563703825166No Hit
GATGGTGGATTCGGAACGGCTACGACGCTGAACGACGTGGCGGG5470.10429496981737894No Hit
CACGTGACGGGTTTGGACCGTGTCAGACGGTGCCAGCGGGTTGG5360.10219763038777899No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5350.10200696316690627No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT5320.10143496150428812No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT5290.10086295984166994No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA5280.10067229262079722No Hit
CCAGTTACCATCTTCGGTGGTGCGTTCGACGTGGCAACGTGCCA5270.1004816253999245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)