FastQCFastQC Report
Sun 21 May 2023
EGAF00007936436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007936436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524474
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC57571.0976711905642607No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT45680.8709678649465942No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG37440.7138580749474712No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG34740.6623779253118361No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA34690.6614245892074726No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG32240.6147111200936558No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT28830.5496935977760575No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT28610.5454989189168576No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC27840.530817542909658No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG27450.5233815212956219No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG26880.5125134897058767No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG24610.46923203056776885No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC24340.46408401560420537No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC24320.4637026811624599No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG23480.44768663460915126No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG22040.42023055480347926No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG21010.4005918310535889No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG17870.34072232369955424No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG17280.3294729576680636No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17110.32623161491322733No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC16110.30716489282595516No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC15760.3004915400954099No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT15350.29267418403962825No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA14720.2806621491246468No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT14040.26769677810530174No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC14030.267506110884429No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG13980.26655277478006534No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG13870.26445543535046545No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT13480.2570194137364293No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC13390.25530340874857477No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC12830.24462604437970234No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG12300.23452068167344806No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC12290.23433001445257534No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG12030.22937266670988457No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT11460.21850463512013943No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT11140.2124032840522123No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG10520.20058191635810352No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9480.18075252538734046No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC9440.17998985650384958No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC9310.17751118263250418No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC9260.17655784652814058No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA9200.17541384320290423No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG9100.17350717099417703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8640.1647364788340318No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT8570.16340180828792278No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA8530.16263913940443187No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC8310.15844446054523198No Hit
GAACGTAACGACCTGGATGTTCAGGGTATCA8160.15558445223214115No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT8070.15386844724428667No Hit
CGTGACACCACTCACATTTCCCAGAGCGCAC7830.14929243394334132No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG7590.144716420642396No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC7430.14166574510843244No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG7380.14071240900406884No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG7150.13632706292399624No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC6960.1327043857274145No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC6870.13098838073956No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG6830.13022571185606913No Hit
CGTGCTCGTGCAGTTATTGACCTGGCAGACG6700.12774703798472373No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT6670.12717503632210558No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6580.1254590313342511No Hit
GAACAGGGTCCGGCAGACGATCCGGGTGAAG6510.12412436078814203No Hit
GAACAGGGCCCGGCGGACGATCCGGGTGAGG6020.11478166696537864No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5950.11344699641926959No Hit
CTGGAAGAGGTCATCGTAGAAAAAACCAAAC5910.11268432753577871No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCTG5740.10944298478094243No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT5670.10810831423483337No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5630.10734564535134249No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5610.10696431090959704No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5490.10467630425912437No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT5430.10353230093388804No Hit
GACGACCCGGGTGAAGGCCCGTCTACTGGCC5270.1004816253999245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)