FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007936475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007936475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498692
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA54761.098072557811234No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA48870.9799635847376738No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT22950.46020389338509543No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC20170.4044580622909531No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA15650.3138209556199017No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA15330.3074041693069069No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC15030.30138843213847427No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT13680.27431761488052747No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA12780.2562704033752296No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT11070.22198070151516366No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10310.20674083402180102No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA9580.19210254024528164No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA9420.18889414708878424No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9380.1880920487996599No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA8650.17345375502314053No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT8150.16342752640908617No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA8130.163026477264524No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6970.13976562687991786No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6830.13695828286798264No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6790.13615618457885828No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6570.13174464398867436No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT6440.12913782454902023No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG6220.12472628395883631No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5990.12011421879637131No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC5900.11830949764584152No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA5680.1138979570556576No Hit
GTGCGGGTAGATCGGCAGGTTGCCCAGCAGAGTGCCGTCGAAGT5500.11028851475459803No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA5160.10347067929704105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)