FastQCFastQC Report
Wed 31 May 2023
EGAF00007936506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007936506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480327
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT58001.2075107166576105No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC31220.6499738719663896No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG29760.6195779125470773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29440.6129157844551733No Hit
CGTTCTCGCCACGAGAACACCTCTCAGGTTC23460.48841726573771616No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT19070.3970211959769074No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17670.36787438557482716No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15210.3166592758683147No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC14860.30937257326779466No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14370.29917118962706657No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC13510.2812667203800744No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT11910.24795607992055413No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC11150.232133525702282No Hit
GAAGGCCCGTCTACCGGTCCGCGCGGCCAGG10720.22318129107878593No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG10560.21985022703283388No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG10270.2138126734495458No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA9760.20319490680307373No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC9640.20069660876860973No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC9390.19549182119680966No Hit
GAACAGGGCCCGGCGGACGACCCGGGTGAGG9120.1898706506192656No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC8950.18633139507044158No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC8890.18508224605320958No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC8450.1759218199268415No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG8340.1736317133952495No Hit
CCTGTAGGTGAAGCGGACTACTTCGAGTATC8330.1734235218923775No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG8250.17175798986940147No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC8070.16801054281770544No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7930.1650958617774974No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6480.13490809386105715No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6470.13469990235818516No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA6390.13303437033520912No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6340.13199341282084914No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6210.1292869232835131No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC6200.1290787317806411No Hit
CGTCACGCACATCGCCAGGGTGTAGGCGGCA6180.1286623487748971No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5980.12449851871745707No Hit
GATGGCGAACACCGTCGTCACCGTGCCCACC5590.11637905010544901No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG5400.11242341155088095No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC5300.11034149652216095No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5200.10825958149344092No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5080.10576128345897691No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5000.10409575143600089No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)