FastQCFastQC Report
Sun 21 May 2023
EGAF00007936522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007936522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536487
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75281.4032026871107781No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT59321.105711787983679No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30340.5655309448318412No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC20200.37652356907063916No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG18940.3530374454553419No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18740.3493094893259296No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG18080.3370072340988691No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA17530.3267553547429854No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17310.32265460300063187No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15720.2930173517718044No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT15060.28071509654474386No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12970.24175795499238564No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC12900.24045317034709135No Hit
GAAGTCTCCATTGCCGAACCGACCACTTACA12540.2337428493141493No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG11760.21920382040944145No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11480.21398468182826424No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC11470.2137982840217936No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC11140.20764715640826337No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10520.19609049240708534No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG10050.18732979550296652No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9500.1770779161470828No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG8990.1675716280170815No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8480.15806533988708021No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC8360.15582856620943286No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8040.14986383640237322No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7810.1455766868535491No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA7800.14539028904707849No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7530.14035754827237193No Hit
TCCATCTCTAGCAGCGAGGAAATCGTCCCAA7480.13942555924001887No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7420.13830717240119517No Hit
ATGGACGAACTGCAGCCGACCATGCAACTGA7290.1358840009170772No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC7250.13513840969119476No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA7090.13215604478766493No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG6960.12973287330354696No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG6930.12917367988413514No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG6610.1232089500770755No Hit
TCCGAGCGTTATCTGGGTTACCTGGAACAGC6570.12246335885119303No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6400.11929459614119262No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6320.1178034136894277No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6300.11743061807648648No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA6110.11388905975354482No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG5810.10829712555942642No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG5800.10811072775295581No Hit
AACGCTACCGTAGCCCTGCAGGGCGAGGTTC5480.10214599794589618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)