FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007936536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007936536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441181
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT74771.694769267035525No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59561.3500128065351862No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC23380.529941225936747No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT15540.3522363837064606No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG13990.31710341107164636No Hit
ACCAGCGGTAATGTTAGCGTTGGTTATAACC13160.29829027088655224No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12330.27947713070145813No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG12130.2749438439098692No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11810.2676905850433269No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA11250.2549973820268779No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA11050.2504640952352889No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG10970.24865078051865336No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT10400.23573091316262487No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC10130.22961097599397978No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG10100.22893098297524145No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG9300.21079783580888573No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG9120.20671787769645564No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA8770.198784625811175No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8760.19855796147159555No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG8740.19810463279243667No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7980.1808781429843987No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC7630.17294489109911804No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7490.16977159034500577No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7470.1693182616658469No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7440.16863826864710857No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG7070.160251688082669No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6910.15662505864939788No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG6860.15549173695150062No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA6760.15322509355570618No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6720.15231843619738838No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC6520.14778514940579943No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6250.14166521223715436No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC6210.14075855487883657No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG6090.13803858280388323No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6040.13690526110598597No Hit
CTGGCATACCGTTCTCTGACCCGCAAAAAAC5880.13327863167271484No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5860.13282530299355594No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG5700.1291986735602848No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG5440.12330540073121915No Hit
CAGGGTGCCCTGACTCTGAGCCTGCCGAAAC5320.1205854286562658No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5260.11922544261878912No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA5240.1187721139396302No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG5150.11673213488341519No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT5110.11582547752509742No Hit
CCGTCTGGCTGGTACAGCTATTTTTTCAAAA5050.11446549148762072No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4990.11310550545014404No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA4970.11265217677098516No Hit
ATGTCTGACGAAGGCCCGGGTACCGGCCCGG4810.10902554733771401No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG4810.10902554733771401No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT4800.10879888299813456No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4640.10517225356486341No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT4540.10290561016906893No Hit
GCCGTCGCGAACAGCAACAACGACACCGCTA4460.10109229545243335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)