FastQCFastQC Report
Wed 31 May 2023
EGAF00007936563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007936563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487058
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG16660.3420537184483162No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG14010.2876454138932119No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13320.2734787232732036No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG12520.25705357472826645No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT10900.2237926489247687No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG10400.21352693108418302No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA9890.20305589888678557No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG9100.18683606469866013No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG8480.17410657457633383No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG7960.16343022802212467No Hit
CAGAGCACGCAGGCCTTCTGCGATGTTTTCGAATTTCGGGTTGG7870.16158239881081923No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT7820.16055582702676066No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7400.15193262404066865No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG6970.14310410669776494No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6600.13550747549573153No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA6540.13427558935486122No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6530.1340702749980495No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA5720.11743981209630064No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5400.11086975267832579No Hit
GGTACGCTCGACGTGGCAACGTGCCAGCAGCAGACGCAGACCTT5400.11086975267832579No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5390.11066443832151408No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT5310.10902192346702036No Hit
GTGACGACGGCGGCGTTCACCGCCGTCCGGACCTTCGCCTTCAC5050.10368375018991577No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5020.10306780711948063No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG4950.10163060662179864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)