FastQCFastQC Report
Sun 21 May 2023
EGAF00007937976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007937976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464891
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT66061.4209782508157827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41220.8866594535063058No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC30760.6616604752511879No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG23740.5106573368811184No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG20990.4515036858102222No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG20520.44139378908174176No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA18650.4011693063535323No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16300.3506198227111301No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG15490.3331963836684298No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT15290.32889429995418284No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG15290.32889429995418284No Hit
CGTATGCCGACTACTAGCACCGCTTCCCAGA14000.30114585999728966No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG13160.2830771083974523No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG12670.27253700329754715No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12370.26608387772617664No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC12120.26070627308336797No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12000.25812502285481975No Hit
GGTCCGGGCATTAACCCAATCAGCAAAACCG11710.2518870014691616No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC11470.2467245010120652No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9170.19725053829822473No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT8270.17789116158411328No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC8180.1759552239127021No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8010.1722984527555922No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7600.16347918114138582No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7530.16197345184139939No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7510.1615432434699747No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC7340.15788647231286473No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG6990.1503578258129325No Hit
CATTCTAACGATCAGCACGCGGGTGATCACA6970.1499276174415078No Hit
CAGTCCCAACCGCAGCGTCGTAAACGTCGCG6850.1473463672129596No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC6780.14584063791297314No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA6590.1417536583844385No Hit
GCTATGCAGACCGGTTGGTACAAAGTAGGCA5880.12648126119886166No Hit
AGCACCCCGCGTGCGGCTGTCACCCAGACGG5590.12024323981320352No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA5520.11873751051321708No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5450.11723178121323062No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG5310.11422032261325774No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC5010.10776719704188722No Hit
AGCAACAAAACCGAAACTCCGTCCGAGACTG4960.1066916761133255No Hit
TTCGAACAGCCGACCCTGACCTTCGGCGCGG4850.10432553007048964No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC4840.1041104258847773No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC4720.10152917565622908No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT4650.10002344635624265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)