FastQCFastQC Report
Wed 31 May 2023
EGAF00007937991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007937991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486785
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27920.573559168832236No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC25760.5291863964583954No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT24590.5051511447558984No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC18610.38230430272091376No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT14840.30485738056842343No Hit
GTCGGTGCAGTTCAGACAAGTTGGGCACAGTTTACCACGAGTTT14080.2892447384368869No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA13820.2839035713918876No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG12860.2641823392257362No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA12340.2535000051357375No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC11670.23973622851977774No Hit
CATGCATTTTGGGCGGCCCAGGGCAACGTCCAGATCAGTGCAGT10970.22535616339862566No Hit
AACTTCGTGCAGGATAGAGGCCTTAGCGGACGGAATGGTACCCA10570.2171389833293959No Hit
GGTTGCGGTCGGCGCTGCAGAGGCGCCAGTGCCAGCGGTGCTGG10380.21323582279651182No Hit
GGTTGCCAGCGGACCAATGATAGTCGGCGGGGTTTTCGGACGGC10290.21138695728093512No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA9980.20501864272728207No Hit
CAGAACGTTCAGGGTGCCGGTAGCTGCCGCCGTTTTAATGGTCA9840.20214262970305166No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC8630.1772856599936317No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT8180.16804133241574823No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG7950.16331645387594113No Hit
CGGAGACAGCTGAGAGTATGGGATGAAATGGTAGTGATCGCCGT7900.16228930636728742No Hit
ACCTGCGGAGGTAACGTTGTTGTTGGTTTTCGGAGCGGTGGTGT7640.15694813932228807No Hit
TGGACGCACTTCGTGCAGGATGGACGCTTTTGCGCTCGGGGTAG7560.15530470330844212No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7350.1509906837720965No Hit
CATGCATTTCGGACGACCCAGTGCCACGTCCAGATCGGTACAGT7250.14893638875478907No Hit
CGGGCAGGACAGACCAGAGGTCAGCTTACAGCGGCATACGTTTG6900.14174635619421305No Hit
ACCAATGATGGTCGGCGGGGTTTTCGGACGAGAGGTTTCCGGAC6620.13599433014575224No Hit
ACCGTGGTCGCCGTTGTCGCCATCGTCACCATCGTCGCCGTCAT6540.1343508941319063No Hit
CAGCACACCGTCGCCCTCGAAAGCACCGCCATCATCTTCGTCCC6340.1302423040972914No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA6250.1283934385817147No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6200.12736629107306102No Hit
TTCCAGTTCGGACATACGGTCGTACGGAATAAAGTGGAAGTGAT6070.12469570755056134No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT5920.1216142650246002No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC5910.12140883552286945No Hit
CAGGTTGCCGTTTACGTAGTGATACAGAGCCACACCTTTTTTCG5870.12058711751594647No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA5760.1183273929969083No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5690.11688938648479308No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG5580.1146296619657549No Hit
TGCACCCAGTTTGCCCTTGTTCGGGGTGCCATCCAGCTCAATCA5360.11011021292767856No Hit
GCCGCTGGTAGCCGGTTTGACTTCGTGCAGAATAGACACCTTCG5260.10805591791037111No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG4910.10086588534979508No Hit
ATAACCGCCAGTCATACGGTCCAGTTCGATGAAACCGGACGGGA4880.10024959684460286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)