FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007937992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007937992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486785
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA55311.1362305740727425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41950.8617767597604692No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT30270.6218351017389607No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT27960.574380886839159No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA26960.5538379366660846No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA23530.4833756175724396No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG15360.31553971465842207No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA15210.3124582721324609No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC11740.24117423503189295No Hit
ATGGCTCAGCGTAACGGTATGTCCCCGCGTC11270.231519048450548No Hit
ACCACTCCGACGAAAAGCCACTTCGCAAACC11100.22802674692112535No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA10970.22535616339862566No Hit
CTGCCGCAGTCTCCAGGCCCAGCGTTCCCGC10820.2222747208726645No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG10130.20810008525324322No Hit
TCCGTGAAAAGCGGCGAACACGAGCTGATCG9280.19063857760613No Hit
GATGGCGAACACCTGGAAGGTGGCGACGGCA8850.18180510903170805No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA8780.18036710251959284No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG8150.16742504391055602No Hit
GACCCAGCTATCGTTCAGCCGAAAGCAGCAA8050.16537074889324854No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG7920.16270016537074888No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT7860.16146758836036443No Hit
GTTGACTCCCTGCACGATGACGGCGATGACG7740.15900243433959552No Hit
TATCACCAGAACCAGGGTGAAAACATTTCCA7570.15551013281017287No Hit
TGGATCGAAGTATCCGGCCAGGAGCTGCCGA7420.15242869028421172No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA7100.14585494622882791No Hit
CTGATCCTGGGCCTGGTTCTGGAACAGGTGC6790.1394866316751749No Hit
TCTGCTACCGGTCGTTCCGGCACCGTACTGG6650.13661061865094445No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA6570.1349671826370985No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG6520.1339400351284448No Hit
ATCGAGAAAACCGATGAACTGGCAACCCTGC6470.1329128876197911No Hit
GAAGCGGAAGCGTGGGAACGTCGTGACGGCG6440.13229659911459885No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6060.12449027804883059No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG6010.12346313054017687No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT6000.12325770103844615No Hit
ATCCCGCTGACCACTACCCCAACCAAATCCC5950.12223055352979242No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5920.1216142650246002No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA5800.11914911100383127No Hit
GTGACCGACCTGATGGCACTGGAAGACTCTG5220.10723419990344814No Hit
AACGTAACCACCAACCAGGACGGCACCGTCT5060.10394732787575624No Hit
CCAGTTACGCTGAACAGCGCCGCACCGGCTT4980.10230389186191029No Hit
ATGTCCAACATGGACATCGATGGTATTAACA4930.10127674435325656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)