FastQCFastQC Report
Wed 31 May 2023
EGAF00007937994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007937994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502682
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT80281.5970335122403427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43730.8699336757632061No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC39280.7814085246736504No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC30190.600578496942401No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC20830.414377280268639No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT19870.39527971958415065No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT18460.36723017732880825No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC16470.3276425254932542No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC16360.32545426333148986No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT16080.3198841414651808No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC15480.3079481660373755No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA14090.28029648962962667No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC14000.2785060933154559No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA13700.2725381056015533No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA12910.2568224046216097No Hit
ATGGCACGTCGCCTGCCGAAACCGACTCTGC12890.25642453877401616No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC12690.2524458802980811No Hit
TGGCGCCCTGCCCCGTGGACCCCGAACCCGC12580.2502576181363168No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA12450.247671490126959No Hit
AGCATCCTGCAGCAACTGAACCCGTGTAAAG11610.23096112452803164No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC11280.22439633804273873No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT11150.22181021003338094No Hit
CACCCAGGTGTGCCGGTCTCTCCAGCCGTTA11100.22081554541439719No Hit
ACTAGCGAACCGTGCGAAGCGCTGGACCTGT10720.21325609431012052No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG10470.20828277121520164No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT10410.20708917367242113No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG10250.20390624689167308No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG9980.19853505794916068No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG9910.1971425274825834No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG9630.1915724056162743No Hit
AGCCCGACCCAACCGATCTCTCACGAAGAAC8980.17864176556948527No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8610.17128124738900538No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC8530.16968978399863133No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC8420.16750152183686706No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG8370.16650685721788328No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG8310.16531325967510277No Hit
GAAAACGTACTGATCGAGCTGAGCGATTCCT8220.16352286336093197No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8020.15954420488499688No Hit
GTCGTGCAGCCGTCTATCCTGCAGCAGCTGA7570.15059222331414293No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG7500.14919969284756565No Hit
ATTCCGGTAGTCCAGCCGTCTATCCTGCAGC7280.14482316852403707No Hit
GAGCAGATCCGTCACGGTGATAACGGCGGTG7190.14303277220986627No Hit
GGTCGTAAAGTACGCGGTGTCATTCCGACTG7100.1412423758956955No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7070.14064557712430523No Hit
TCCCCGACCCAGCCGATCAGCCACGAAGAAC6900.1372637174197604No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6860.1364679857245734No Hit
TCTATCCTGCAGCAGCTGAACCCGTGCAAAG6860.1364679857245734No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT6580.13089786385826427No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6470.12870960169649998No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG6310.12552667491575192No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT6280.12492987614436166No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6210.12353734567778438No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG6140.12214481521120707No Hit
CAACAGTACCAGCAGGTGCTGCAGCAGCAGA6100.12134908351602008No Hit
ACTACCGACCCGGATGCAGCGGCTTCCGCGA6020.11975762012564604No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG5910.11756935796388174No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5890.11717149211628822No Hit
GCCCCGTTCGACACCTTCACCGGTGACTACG5860.11657469334489796No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5840.11617682749730446No Hit
TCCGTCAACGGCCAGGCCAAATTCGGCGGCA5840.11617682749730446No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5720.1137896324117434No Hit
GGCGGTACCTACCGTAGCTATGAACAGCTGA5700.11339176656414991No Hit
AAATCTGGCATGGAATCCCTGAAAAACATTC5670.11279496779275963No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5540.11020883978340183No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA5510.10961204101201158No Hit
CCAGTTGTGCAGCCGTCCATCCTGCAGCAAC5430.10802057762163754No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5400.10742377885024727No Hit
GCGTCTGAGCTGGCCAAAGAAGGTAAAACCA5390.10722484592645051No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5370.10682698007885702No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG5370.10682698007885702No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA5280.10503658376468622No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5250.10443978499329595No Hit
ATGCTGGTGCAAGTACAGATCCCGTTCGTTC5230.10404191914570246No Hit
CCGCCAGCCCCGCAGGCGCCTTACCAGGGTT5160.10264938867912518No Hit
GCCGCGACCGGCGAGTACGAAGACCTGCTGA5080.10105792528875114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)