FastQCFastQC Report
Sun 21 May 2023
EGAF00007938003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448046
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT51681.1534529936658289No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT40970.9144150377416606No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT40580.9057105743606683No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT31050.6930091999482196No Hit
AACGGTTACGCTGCTCTGTTCAGACGGACCCGGGCGTTCCGGGG29590.660423260111685No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC24520.5472652361587873No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG23290.5198126978033506No Hit
GACGCTGCTCTGTTCAGACGGACCCGGACGTTCAGGGGTGGACT22640.5053052588350303No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT22290.4974935609290117No Hit
GCGGACGGACATCTGAGACGGGGAACGCGGTGGGTTCGGGGTCC22150.4943688817666043No Hit
AACGGTGACGCTAGACTGTTCGGAAGGACCCGGACGTTCTGGAG22020.49146739397294026No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA19820.44236529284939496No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT18580.4146895631252148No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG18110.4041995687942756No Hit
CGGCGCACCGTGGTTGATCGGCTGGGTCAGCGGCAGGTCGAAGT17750.3961646795195136No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA14910.3327783307963914No Hit
GTTCTGGGACGCAGCCATGTAGTCCTGCGGTGCCGCACGACGCT14020.3129142989782299No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG13990.3122447248719998No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG13890.31001281118456586No Hit
CACGCTAGACTGCTCGGACGGACCTGGACGTTCTGGGGTAGACT13860.3093432370783357No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT13330.29751409453493616No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT12930.2885864397852006No Hit
CTGTTCGGTTTCCAGTTCGCCTACGCTCACTTTGGTGTTGTTAC12840.2865777174665101No Hit
AACGCTAGACTGTTCGCTCGGACCAGGACGTTCTGGAGTGGACT12660.28256027282912916No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA12440.2776500627167746No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG12200.2722934698669333No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG11860.26470496332965815No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG11770.2626962410109676No Hit
GTCGGTGCAGTTCAGACAAGTTGGGCACAGTTTACCACGAGTTT10820.2414930609803458No Hit
CTCCTGACCTTCTTCACCTTCATCACCATCACCGCCTTCATCAC10690.23859157318668173No Hit
TTCCTGGCCTTCTTCACCCTCATCACCGTCACCACCTTCATCGC10150.22653923927453876No Hit
TTCCGGCAGAACCGGCCACAGTACGCAAGGCAGCGGAGCCTGGG9510.21225499167496195No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG9460.211139034831245No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT9310.2077911643000942No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA9190.20511286787517355No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT9040.2017649973440227No Hit
GGCCTGTTCCTGGTTGGTAATGGACTGCGGAGTTTCGACTTCAA8930.19930989228784546No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG8530.19038223753810993No Hit
CTCACCTACAGAAACTTTAGTGTTGTTGCCTTTGTCACCGAACT8340.18614160153198556No Hit
CAGGAAGTTCTCGATAGAAGATTCGGAGCGGGAGTGGTAGTTAT8250.18413287921329505No Hit
TTCCTGACCTTCCTCACCTTCGTCGCCATCGCCACCTTCATCAC8010.17877628636345377No Hit
ATAATCCCAAGATTCGTCCAGGTCCGCCGGGTCGATGGACGGTG7930.17699075541350665No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT7640.1705182057199484No Hit
TTGTGGCACCGGTGGCAGCAGGGTGGCCATGACACGCTGTTCCA7600.16962544024497486No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG7380.16471523013262032No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG7260.16203693370769964No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG6930.15467161853916786No Hit
AATCGGCAGGCTTGGGGACGGGGTCGCCAGAGAGTTGTTTGGCA6820.1522165134829906No Hit
CATGCATTTCGGACGACCCAGTGCCACGTCCAGATCGGTACAGT6740.1504309825330435No Hit
CATGCATTTTGGGCGGCCCAGGGCAACGTCCAGATCAGTGCAGT6470.14440481557697202No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG6420.14328885873325506No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC6300.1406105623083344No Hit
TTCCTGGCCCTCTTCGCCTTCATCACCATCGCCACCTTCGTCGC6280.14016417957084765No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG6170.13770907451467038No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA6050.1350307780897497No Hit
GTGGGAGTTACGGCATACCGGTGCCGCGTTAGATGGACCCGGGG6020.13436120398351956No Hit
ACCGCCATGAGTACCTTTGCAGCCAATACAAGACGGGCGTTTTT5870.13101333345236874No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5780.12900461113367825No Hit
AATAGAGGATTCGGAACGGCTGTGGTAGTTGTGCACGTGACGAG5680.12677269744624436No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG5680.12677269744624436No Hit
GATGCGATGCGGGGAGTCGCAGATACGACCGGTAGAGCTGGACT5450.12163929596514644No Hit
CTCCTGGCCTTCTTCACCTTCATCGCCGTCACCGCCTTCGTCAC5410.1207465304901729No Hit
CTGAACAACATCGGTACCCTGAGAAGGCGGTGCGCCCTGAAACA5260.11739865995902207No Hit
GAACACTACAACTTTTTTGTTGCCGATCAGATAAATACCGGCCG5220.11650589448404852No Hit
GTCGCCCGGTACTACCTGGGAGGTGTGGCCAGTTTCAGCAGCGG5180.11561312900907494No Hit
TGGCGGCGGTTCCTGGTAGCCCTGATACGGTGCCTGCGGCGCCG5090.11360440669038448No Hit
GTTCGGCAGGGTCGGCTGGCCGATCTGGTTGGATTTCGGGATGT5070.1131580239528977No Hit
GTGCTGGGAACGTGGGGTCCACGGGCCTGCATAACCCGGGTAGG5040.11248844984666753No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT5020.11204206710918076No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG5020.11204206710918076No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA4990.11137249300295059No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT4950.11047972752797704No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT4950.11047972752797704No Hit
TGGACGCACTTCGTGCAGGATGGACGCTTTTGCGCTCGGGGTAG4840.10802462247179977No Hit
AACGCTGGATTCGGTACGGGAGTGGTAGTTTTTTACGTGACGAG4730.1055695174156225No Hit
TTCTTGACCTTCTTCGCCCTCATCACCATCGCCGCCTTCATCGC4670.10423036920316217No Hit
CTGGATGGACGGACGCGGGCGTTTGCTAGGACCCTCGACGTAGT4660.1040071778344188No Hit
TTTCACGCATTTCTTGCCGTCCACTACGTAAACGCCGGACGGCA4580.10222164688447168No Hit
GTAAGCGCCCTGGGTGGTCTGCATGATAGCACCCGCTTTCGGGG4510.10065930730326796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)