FastQCFastQC Report
Sun 21 May 2023
EGAF00007938004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448046
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT50731.132249813635207No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT42850.9563750150654174No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT41380.9235658838601394No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC35480.7918829763015405No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA32750.7309517326345956No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC31800.7097485526039736No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC26500.5914571271699781No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA25130.5608799096521339No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA23200.5178039754846601No Hit
ATGGAGCCAGTTCAGCAGCGCCCGGTTATGT22340.4986095177727287No Hit
CTGGAACGTCCGGTGTACCCGAAACCGGTTC22020.49146739397294026No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA21000.4687018743611147No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG20590.45955102824263583No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA18500.4129040321752677No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA17630.39348638309459294No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT16280.3633555483142356No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG16240.3624627828392621No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG14800.33032322574021417No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG14720.32853769479026707No Hit
GCACGTCGTCAGGAAGCAGAACGCCAGGCCG14080.3142534471906902No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG14050.31358387308446006No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG13930.31090557665953944No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA13790.307780897497132No Hit
ATGGAACCTGTGCAGCAGCGCCCGGTAATGT13520.3017547305410605No Hit
CTGTCTGCTGAGCAGCTGAACGTCACTGACG13400.2990764341161399No Hit
ATGGAACCGGTCCAGCAGCGTCCGGTTATGT13270.2961749463224758No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC13010.2903719707351477No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG12800.2856849519915366No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG12570.2805515505104386No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT12340.27541814902934075No Hit
TCCTCCAACAGCCCGCACGTGGTTAAAACCG11880.26515134606714486No Hit
GCTATTAAAGATCTGGTGATGACGAAACCGG11870.26492815469840153No Hit
GCACCAACCTGCAACATCAAAGTAACCGTTT10520.23479731991804415No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG9970.22252179463715777No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG9740.21738839315605987No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC9640.21515647946862598No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG9600.21426371399365243No Hit
GAAAGCGTCGAACGTGCTATGGGTCGTGTTG9570.2135941398874223No Hit
GTGGTAGAAATGGAGGGTTACCGTTACGTGG9260.20667520745637724No Hit
GCCGGTGATTCCCCAACTCCGGGCCCGTCCA8610.19216776848805703No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG8460.1888198979569062No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8400.1874807497444459No Hit
GCACCGACCTGCAACATCCGTGTGACCGTTT8360.18658798426947232No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8340.18614160153198556No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT8300.185248836057012No Hit
GTGCGTGCATCTAACCTGTCCGCCGAACAGC8200.18301692236957812No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC8110.18100820005088764No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7480.16694714382005418No Hit
CCGTGGCAGCCGCTGCCGGGTCCGCAGGTGA7340.16382246465764677No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT7300.1629296991826732No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC7240.16159055097021288No Hit
GATGGTTACATCTTCAACCCGAAAGACATCG6850.15288608758922073No Hit
ACCCAGGGTGAAGTTAACGTAACTGGCGTTA6790.15154693937676042No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG6730.1502077911643001No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT6550.14619034652691912No Hit
GCTCCGACCTGCAACATCCGTGTTACCGTTT6510.14529758105194557No Hit
ACCCAGGGCGAAGTAAACGTGACGGGTGTTA6470.14440481557697202No Hit
GGTGGTGATAACCATGGCCGTGGCCGTGGCC6420.14328885873325506No Hit
CGCGGCCGTGGTAAAGGTAAGTCTCGTGATA6360.14194971052079475No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG6080.13570035219597987No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA6030.13458439535226294No Hit
ATGGGTCGCGTAGCAGACACTATCGCTCGTG5820.1298973766086518No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG5720.1276654629212179No Hit
GGTAACGATAATTCTACTGCAACTCTGTGTC5640.12587993197127081No Hit
CCGACCAAGTCTCACTTCGCCAACCTGAAGG5480.12230887007137661No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5460.12186248733388984No Hit
GCTATCAAAGATCTGGTTATGACCAAACCGG5420.12096972185891626No Hit
AGCCTGGGTTCCCTGAGCTCTAACCCAAGCT5270.11762185132776545No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC5260.11739865995902207No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC5260.11739865995902207No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC5220.11650589448404852No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG5190.11583632037781835No Hit
TCCTACACCAACGGCAAACTGACTATCACCC5190.11583632037781835No Hit
GGCTACACTTCCGGCCGTAACCTGTACACCA5130.11449717216535803No Hit
CCGGAAGCACCGATCCTGTTCCCGGATGACT5090.11360440669038448No Hit
GTGGCACAGTCCCAGTACTTCGACCTGCCGC5070.1131580239528977No Hit
GCAATCAAAGACCTGGTGATGACCAAACCGG4860.10847100520928654No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC4830.10780143110305639No Hit
CCGATTCACGAACCGGAGTCCCACAACAGCC4700.10489994330939234No Hit
GTTGCACGCGTCGCCGACACCCTGCCAACGG4680.10445356057190558No Hit
CTGGGCCATCACGCAGTGCCGAACGGTACCC4600.10266802962195845No Hit
CCGAAACGTCTGAACCTGTACATCGATGGTG4550.10155207277824153No Hit
GGCTACCAGGAACCACCGGCACCACAAGCCC4550.10155207277824153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)