FastQCFastQC Report
Wed 31 May 2023
EGAF00007938005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469860
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT25540.5435661686459796No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT17750.3777721023283531No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15620.33243945004895076No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG13900.2958328012599498No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC13440.28604265100242626No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG12660.2694419614353212No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC12200.2596518111777977No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT11490.2445409270846635No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG11380.24219980419699486No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA11130.23687907036138425No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT11060.23538926488741327No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC9920.21112671859702892No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG8760.18643851359979569No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA8740.18601285489294683No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG8680.1847358787724003No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA8210.17473289916145235No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC8020.17068914144638828No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA7850.16707104243817306No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT7770.16536840761077767No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA7660.163027284723109No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7610.16196313795598688No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG7320.15579108670667857No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG7080.1506831822244924No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG6870.1462137658025795No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6710.1428084961477887No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG6380.1357851274847827No Hit
GCGAATGTATGCCAGCCACGCTTCGTCTTCAGCCAGGATATCGC6270.13344400459711403No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6210.13216702847656747No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT6170.1313157110628698No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG6140.13067722300259652No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG5680.120887072745073No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA5650.12024858468479972No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT5380.11450219214234027No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG5360.11407653343549141No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG5290.11258672796152044No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG5230.11130975184097391No Hit
GTCGGACGGGGTTTTTGCACCACCCATGCCTGCGGAGCCCGGCT5120.10896862895330524No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC5030.10705316477248542No Hit
CGGCTGACCGTCATCTGCTACATTGGTACAGACGCGTGCGCCGT4930.10492487123824118No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT4900.1042863831779679No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA4770.1015196015834504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)