FastQCFastQC Report
Wed 31 May 2023
EGAF00007938031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442070
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT27590.624109303956387No Hit
TTCCATGGCTTTAGCAGTGGTAGACGCCAGAACCATACGGTTTT15170.34315832334245705No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG12340.27914131246182733No Hit
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA11760.2660212183590834No Hit
TTCCATAGCTTTTGCGGTAGTGCTGGCCAGAACCATGCGGTTTT11330.2562942520415319No Hit
ACGGCGGAAGGCATACGGGTTGTCTTCGTCTGGCGCATCAATAT10270.23231614902617234No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG10110.2286968127219671No Hit
CTGGACGGAAACAGAACGACGGCTACGTTTGGAGTGGCGGGTAC10000.22620851901282604No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA9940.22485126789874907No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT8710.19702762006017144No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT8370.18933653041373538No Hit
CAGGACCATACGGTTTTCGTGACGGATCAGCGGGTTGGTAGTGG8350.18888411337570973No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG8040.1818716492863121No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG7810.17666885334901714No Hit
GAAGATGTAATCCCAGGACTCATCCAGGTCAGCCGGATCGATAG7670.17350193408283757No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG7100.16060804849910648No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6890.15585766959983713No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT6500.1470355373583369No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG6200.14024928178795212No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG6080.13753477955979823No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG5930.13414165177460582No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG5580.12622435360915693No Hit
GGACGGGTGGGTACCGATAGTACGCATGGCCTGCACCATTTGGC5360.12124776619087474No Hit
CGGCTTAACCGCGATGGAACGGCGGCGACGACGGCCAGCGTATA5350.12102155767186192No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA5330.12056914063383628No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5060.11446151062048998No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC4970.11242563394937453No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG4880.1103897572782591No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT4830.10925871468319497No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG4820.10903250616418214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)