FastQCFastQC Report
Sun 21 May 2023
EGAF00007938035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498246
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA46040.9240415377143019No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA41370.8303127370816825No Hit
TTTCAGGGACGGTACGCCGACACGAGAACCGCGACCTTTAACTG37170.7460170277332884No Hit
GGTAGAAGCGGCGCGGGAGCTACCAGACAGGCGACCCGGTGCGG32980.6619220224547713No Hit
TACACCGACACGGGAACCGCGACCTTTAACCGGGGATGGCGGAG26470.5312636729647604No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT20960.42067573046246237No Hit
AGCGGAAGAGCCGAACAGGCGACGGCTTGCACCTGGTTTGCGGG20470.410841231038483No Hit
GGACGCCATAGCACCACCGCCAGTCACACGCGGGGTTTTACGTT20340.40823207813008033No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA15160.30426736993372755No Hit
TGGCTGGGTCGGAACGTGTTTTTTCGGGACGCTGCTGAAGGCAG15140.3038659617939733No Hit
GGCCGGGTAGGTTGGAGTGGTGGAAGCTGCACGGGAAGAGCCGG12280.24646459780911437No Hit
TTTAACAGTAATACCGTTGCCATTGGTGTCTTTGGTAACGCTGG11580.23241531291771536No Hit
AACCGCCGGCGGGCCGGTATCGCTTGGGGATACGGTTGGCGGTG11140.22358433384312165No Hit
ATTACCGTTGTCGAAGGTCACGTTAGTACCGGAAGTTACCGTCT11080.22238010942385888No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC11030.22137658907447327No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG9480.19026745824351826No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT9460.189866050103764No Hit
AGGGTGGGTCTTCGGGGTCGGCCACGGCAGTTTACGCGGTTTTT9360.18785900940499273No Hit
TGCACGTTCCGGAGACGGGTTACGGGTGCGTTTGTTAGACGGCG9350.18765830533511557No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT9210.18484844835683578No Hit
AGACGCTAGATGAGGAGCGTACGGAGCTGAACACAGATTTAAAT9170.18404563207732727No Hit
CAGACCCATTGGTTCGTTCATGATGTCGTTGTCCAGGCCCGGAC8850.17762310184125915No Hit
TTTCAGGATAGACGGGCTTGCAACCGGCGGAGCGTTGGATACAG8080.1621688884607202No Hit
TGGACCGGTAACCGGACGGCCACGACGGCCGTTATCGAACAGGG7750.15554565415477495No Hit
TGCGTCTTCGAAATCCTCGTCATCGTCATCTTCTTCTTCACGCA7500.15052805240784672No Hit
CCAAACTTCGTCGGCCGCATCACGAGAAGCGGTCTGAGTGACGG7410.14872171577895257No Hit
CGGATGAGTTTTCGGAGTCGGCCACGGCAGTTTACGCGGCTTCT7340.14731678728981268No Hit
CAGGCTAAACGCTGCGTGACGCTGTTGCTGCTGCGGAATGTTCA7000.14049284891399028No Hit
GAACGGACGAGTCGTCTTGCGCGGTTTTTTACGCGGAGACAGTT6900.138485808215219No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT6870.13788369600558759No Hit
CATCGGAGAAGACAGTTTTGCTTTGCGAATACGGCTCGGGGTGG6690.13427102274779928No Hit
TTTACGGGTCGGCGGCGGCGGCTGCTGTTGACGCTGCTGCAGGT6500.13045764542013383No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG6430.12905271693099393No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC6350.12744708437197688No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6140.12323229890455718No Hit
AGTCACGTTACGAGAAGAGGTGGTTTTGCTGGAGGCCGGCTGCG6040.12122525820578589No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT6010.12062314599615451No Hit
ACCGCTGAACAGATCGCCGCGGGTTTCGGTCAGTGGACGCACGT5400.10838019773364964No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5390.10817949366377251No Hit
GGCGATCAGGTCGGACAGGTTTTTCTTGATAACTTCGCCAGACG5360.10757738145414113No Hit
CGTACTCCTGGCGACAGAGAAAATGGCGGTGACGTTATTTAAAT5290.10617245296500122No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG5120.10276048377709003No Hit
CGGGTGGGTTTTCGGGGTCGGCCAAGGCAGTTTACGCGGCTTTT5060.10155625935782725No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5010.10055273900844161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)