FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007938036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498246
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT94871.9040795109243225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41330.8295099208021741No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG36080.7241402841166813No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG34060.6835980620015013No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG25590.513601714815573No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT21400.429506709537056No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT20700.415457424645657No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG19680.3949856095181898No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC16090.32293284843230047No Hit
GAAGATTTCGACATCTACGTGGACTCTTTCC15660.31430257342758394No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG13260.266133596657073No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA12010.2410455879224319No Hit
GGTCTGGTTACTGCTAAAGCTGTCATCGATG11900.23883784315378345No Hit
GAAGCAGGTGGCACCAACACCACCCCGGCGA11050.2217779972142275No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG10570.2121442018601253No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA10420.2091336408119684No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10200.20471815127467158No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG9660.19388013150130656No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA9510.19086957045314965No Hit
ATGTCCAACATGGACATCGATGGTATTAACA9180.18424633614720437No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9150.18364422393757301No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT8510.17079916346543675No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA7990.16036255183182604No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7800.1565491745041606No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC7410.14872171577895257No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT7400.14852101170907545No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7300.14651397101030417No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT7110.14270059368263868No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA7030.14109496112362166No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG6950.1394893285646046No Hit
CCAGCGGCTGAAGCTCACGCCGCGCGTTCCG6860.13768299193571049No Hit
ACCGGCCCGACTCTGACTACCCACTCTTGCT6770.1358766553068163No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6730.1350738390273078No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG6410.12865130879123968No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6340.12724638030209978No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG6310.12664426809246837No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG6240.12523933960332848No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6110.1226301866949258No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6070.12182737041541729No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG5980.12002103378652312No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5690.11420061576008639No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC5630.11299639134082361No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5510.11058794250229807No Hit
CTGGCATACCGTTCTCTGACCCGCAAAAAAC5410.10858090180352678No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5400.10838019773364964No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5400.10838019773364964No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5130.10296118784696714No Hit
CGCCTGGACGCAGAACAGCGCGCCCAGCAGA5000.1003520349385645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)