FastQCFastQC Report
Wed 31 May 2023
EGAF00007938038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503997
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT101442.0127103931174193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40030.7942507594291236No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG36900.732147215161995No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG33310.6609166324402724No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG26270.5212332613090951No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21580.42817715184812605No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG21320.42301839098248595No Hit
ATCCAGGGCAAACTGGAATACCGTCACACCT20970.4160739052018167No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC16130.32004158754913226No Hit
GAAGATTTCGACATCTACGTGGACTCTTTCC15730.31210503237122444No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG13580.26944604828997No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA11530.22877120300319248No Hit
GGTCTGGTTACTGCTAAAGCTGTCATCGATG11520.2285727891237448No Hit
GAAGCAGGTGGCACCAACACCACCCCGGCGA11240.2230172004992093No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA10810.21448540368295843No Hit
ATCGAAGTGATTGACGTTGAAACCACTGAAG10790.21408857592406302No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT10550.20932664281731833No Hit
CAGCGCGGTGATCCGTTCGACAAAAACTACG10020.19881070720659053No Hit
ATGTCCAACATGGACATCGATGGTATTAACA9790.19424718797929352No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9270.18392966624801338No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA8890.17638993882900098No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT8280.16428669218269157No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT8110.16091365623208076No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG7890.15654855088423145No Hit
AACGACCTGTACCGCCGCGTGATCAATCGTA7720.15317551493362064No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC7700.15277868717472526No Hit
GCTTGGGCGCTGAAAAACCCGCACCTGGCAT7630.15138979001859137No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC7580.1503977206213529No Hit
TTCACTCCGATCAAGAAACCGGGTACCTCCG7340.14563578751460823No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA7330.14543737363516052No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG7280.14444530423792204No Hit
CCAGCGGCTGAAGCTCACGCCGCGCGTTCCG7140.1416675099256543No Hit
ACCGGCCCGACTCTGACTACCCACTCTTGCT6840.13571509354222347No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG6650.13194522983271728No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC6480.12857219388210644No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC6270.12440550241370485No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG6260.12420708853425715No Hit
CCGACCAGCGGTCCGCTGAACATCCCGCAAC6060.12023881094530325No Hit
GTTGCGACCCCACACGCCTCCGCGCGCGCTC6020.11944515542751247No Hit
CTGGCATACCGTTCTCTGACCCGCAAAAAAC5890.11686577499469243No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG5880.11666736111524473No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG5850.11607211947690164No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5680.11269908352629084No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5540.10992128921402311No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA5410.10734190878120306No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG5360.1063498393839646No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG5360.1063498393839646No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA5310.10535776998672611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)