FastQCFastQC Report
Sun 21 May 2023
EGAF00007938090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544999
Sequences flagged as poor quality0
Sequence length31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA99871.83248042656959No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA59711.0955983405474137No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA57091.047524857843776No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG21570.39578054271659213No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA20430.37486307314325346No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19290.3539456035699148No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG18780.3445877882344738No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG17660.3240372918115446No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA15490.28422070499211927No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA13660.25064266172965455No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC13410.24605549734953644No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG12860.23596373571327656No Hit
GCTCTGGTCTCCGCCATGGAACGCACCGAAC12490.22917473243070172No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC12080.221651782847308No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC11580.2124774540870717No Hit
GTGCCGGTCAGCGGCTCTGCTCCGGGTCGCC11320.20770680313174886No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG11030.2023856924508118No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT10990.20165174614999293No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA10560.19376182341618975No Hit
AGCCCGGCTAAATCTGCGCCGCCGTCTCCGG9430.17302784041805583No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT9190.1686241626131424No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9040.16587186398507153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8760.16073423987933924No Hit
CGCGAAATGATCCGTTATATGGCACTGGTTT8630.1583489144016778No Hit
CTGGTGGACTACCAGGATAAGATGATCAAAG8430.15467918289758328No Hit
TCTATCCTGAAACCAGGTGCTTCTGCTGCCC8350.1532112902959455No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT8180.15009201851746518No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA8110.14880761249103208No Hit
CCGGATCCGGCTGTTGCCCCGACCAGCGCGG7950.1458718272877565No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC7850.14403696153570925No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7790.14293604208448088No Hit
GCGCCGGTTTGCCGTAACTCCCACACCGCCA7700.14128466290763836No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC7620.13981677030600057No Hit
GCTCCGACCAGCGCGGCGTCTCGCAAACCTG7580.13908282400518165No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG7540.13834887770436274No Hit
GAAGAGGAAGATGACGACTCCAGCGATACTG7530.13816539112915804No Hit
GAAGAGGCTCCGGCAGTTGATCCAGTGCAGG7450.13669749852752022No Hit
GATATTCGTGTTTACGGCATGAACGGCTGCA7300.13394519989944936No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA7100.13027546839535484No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC7090.13009198182015014No Hit
CAGACCCCAGAACAGTCTACCCCGTCTCGCA6950.12752316976728398No Hit
GCCGAAGCTGATTATTTCGAATACCACCAGG6950.12752316976728398No Hit
GGTTACCAGGAACCGCCGGCACCACAGGCGC6920.1269727100416698No Hit
GCGAACGAAGCAGGCCAGGTGGCCGGCGGTG6890.12642225031605564No Hit
GCACTGGTAAGCGCTATGGAACGTACTGAAC6740.12366995168798475No Hit
TCCTACACCAACGGCAAACTGACTATCACCC6680.1225690322367564No Hit
TCTACTGCCAAGAGCTCCGTATCCAACGCGC6660.12220205908634696No Hit
CGTGAACGCCAGAAACGTGTTGACGATGAGG6410.11761489470622881No Hit
CTGAACCTGCCGGAAGAAGTAGTGCAGTACA6230.11431213635254375No Hit
GTGGCAACCAACGAAATGAAGGTTATCAACG6220.11412864977733905No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA6180.11339470347652013No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT5970.10954148539722092No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT5800.10642221361874059No Hit
GTGCGTTATTTCCGTGGCCGCGGCAGCGGTT5780.10605524046833113No Hit
GTGGAAGCGTGGATCCCGCGCGCTCCGCGTG5540.10165156266341772No Hit
GCCGTCCGTTATTTCCGCGGCCGCGGCTCCG5490.10073412978739411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)