FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007938099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389925
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA52491.3461563121113034No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA47891.2281849073539783No Hit
CGGCGGAACCGGGACAGTAGCCTGCGCGGTGGTCACCGGTGGGG28890.7409117137911138No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC26910.690132717830352No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA23950.6142206834647689No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA19020.4877861127139835No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA18950.48599089568506765No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18510.47470667436045394No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT18190.466499967942553No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT14480.37135346541001474No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC13690.35109315894082194No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13500.3462204270051933No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG12770.32749887798935695No Hit
AGACGGCGGCGGGGTAGGTTTGACCGGAACTGGTTTCGGCACTG12030.30852086939796114No Hit
AACGCAGGCACCGCGACGACGACGGGACGGTGGTGGGGTCGGTT10950.2808232352375457No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT10650.2731294479707636No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT10440.26774379688401617No Hit
ACGAACAATCACCTTAATTGCTTTGCCGTTAACTTTAACTACGT9940.25492081810604605No Hit
GTCACCCGGAACGACCTGGCTGGTGTGGCCAGTTTCTGCTGCGG9920.2544078989549272No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA9810.2515868436237738No Hit
CATGGTGTCGCCCGGCACTACCTGGGAAGTGTGGCCGGTCTCAG9770.2505610053215362No Hit
ACGACGACCGCCGTCGCCCTGACCACGTGGGCCGGTGGACGGAC9240.23696864781688787No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT9090.23312175418349684No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA8720.22363274988779894No Hit
CATGGTGTCACCCGGGACAACCTGGCTGGTGTGACCAGTCTCAG8450.21670834134769507No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8380.21491312431877926No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG7660.19644803487850226No Hit
GCGCACTACAACTTTCTTACCACCCACAACATAAATACCAGCCG7190.18439443482721035No Hit
ACCCAGCGTGATAGAACGCACGCGGTCAGAGTAACCACATTCCT6920.17747002628710648No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT6530.1674681028402898No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6300.16156953260242354No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT6110.1566968006667949No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT6000.15387574533564147No Hit
CAGAGCGAAAGTAATGTTTTTGCCATCACGCTTAACCTTCAGGT5680.14566903891774058No Hit
GGTGGACGGACCTTCGCCCGGGTCATCCGCCGGGCCCTGTTCGA5680.14566903891774058No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG4730.12130537923959736No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA4710.12079246008847855No Hit
CGGCGGCGGTTCCTGATAACCCTGGTACGGGGCTTGCGGAGCCG4690.12027954093735975No Hit
CGGGCCTTCGCCCGGGTCATCAGCCGGGCCCTGTTCGATCGCAC4480.1148938898506123No Hit
GGTACCGCTGTTCAGGGTGTACTGCTGGAACAGCTGCTTCCACG4450.11412451112393408No Hit
CAGGGTTTTCAGGAAAACCAGCGGCGGACGCTTCTGCAGACCTT4300.11027761749054306No Hit
GGGCACTATCGAGCAACGCCTTCATTGCATCCGAGTAAGTATGA4090.10489196640379561No Hit
CATGGTATCACCTGGAACAACCTGGCTAGTGTGGCCGGTTTCAG3970.10181445149708278No Hit
TGTGACATTTAAATTGGAGTATCAGGTAGACGGGCCTTCGCCCG3960.10155799192152336No Hit
GTTGACCCATTCGCCGTTAGCGTTAACACCATAGCCGTCAACGG3910.10027569404372635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)