FastQCFastQC Report
Wed 31 May 2023
EGAF00007938100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389925
Sequences flagged as poor quality0
Sequence length31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT121093.105469000448804No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56121.4392511380393664No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC37120.9519779444765019No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT28290.7255241392575495No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA24730.6342245303584022No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG21980.5636981470795666No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT20600.528306725652369No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG18530.4752195935115728No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT17990.46137077643136504No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC15220.39033147400141055No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG14560.37340514201449No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG13430.3444252099762775No Hit
GCAGAAGCGCAGTCCACTCCGGAGCGTCCTG12090.31005962685131755No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG10930.28031031608642687No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG10780.27646342245303585No Hit
GACAAAGCTGAAGCACAGTCCACTCCGGAAC10490.26902609476181316No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA10360.2656921202795409No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC9880.25338206065268964No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG9870.2531256010771302No Hit
GACAACATCCGCGTCTATGAAGAAAACGGTA9800.2513303840482144No Hit
CGCCGTCCGCCACCTGGTCGTCGCCCGTTCT9550.24491889465922934No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT9460.24261075847919472No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC8670.22235045201000192No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8480.2174777200743733No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG8230.21106623068538824No Hit
TCCTACACCAACGGCAAACTGACTATCACCC7060.18106046034493814No Hit
GATGCGGCCTCCAATTCTGCAAACCGTCAGG6960.1784958645893441No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6720.17234083477591844No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6700.17182791562479963No Hit
TCTCCGCCGCGCCGTCCGCCGCCGGGTCGTC6690.17157145604924023No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6490.1664422645380522No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA6230.1597743155735077No Hit
CCACGTCGCCCGCCGCCGGGCCGTCGTCCTT5900.15131114958004743No Hit
GGTGACGTTGCTAAAGCGATCAATGACGCGG5720.1466948772199782No Hit
ATTGAGCAGAAACAGCTGCTGCAGGGTGATG5620.14413028146438417No Hit
ATGACCCAGGGTCGCCGCGGTAACGTTTACT5310.1361800346220427No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA4830.12386997499519137No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT4760.12207475796627557No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC4630.11874078348400334No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC4550.1166891068795281No Hit
GGCACTTCCGCTTTCGGTGATTCCGCGGACC4320.11079053664166186No Hit
CAGAAACAGCTGCTGCAGGGTGACGTGGAAG4280.10976469833942425No Hit
ATGGCACTGATCAGCCAGTCCATTAAAAACC4230.10848240046162723No Hit
AACGGTGACATGGCTACCGGCTGGGTGAAGG4200.10771302173494904No Hit
GAACGCGCACGTGGTCGTGGCCGTGGCCGTG4040.1036096685259986No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG4010.10284028979932038No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC3980.10207091107264218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)