FastQCFastQC Report
Sun 21 May 2023
EGAF00007938101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419395
Sequences flagged as poor quality0
Sequence length44
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGTCGGCACGTACGATGTAATCGCGTTTCCCCAGTAGAA30750.733199012863768No Hit
ATCGGTGCAGGATTGCGTAATTTAGTAGCGCCGTCCGTAAGAAA27290.6506992214976335No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC22150.5281417279652834No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT21970.5238498313046174No Hit
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT21400.5102588252125084No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT18830.4489800784463334No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC18150.4327662466171509No Hit
TTTGTCGATGGTCTGCTCCAGGTTACCAAAGAAGCCTGCTTTTG17670.421321188855375No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT15950.38030973187567807No Hit
GTAGTTCGGGTCGATATCTGCGACACCTTCGGCGTCCAGCATTT15540.3705337450374945No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC15100.36004244208919994No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA12430.2963793082893215No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11640.2775426507230654No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG10010.2386771420737014No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC9960.23748494855684973No Hit
GTCGTGACGTTTCAGCGGGAATGGGCCACCCAGATCGTCGATGG9600.22890115523551782No Hit
TGGTGCCTGAGACTCGTTCAGTTTCTTAGCTTCGCCCAGGACGT9100.2169792200670013No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT8500.2026728978647814No Hit
GCTAGTTATAGCGGGGCCGAGGTTACTAACCAAAGTGTCGGCAC8410.2005269495344484No Hit
CTGACCAACACCGGAGAAGTCGCCAACGCCTTTGTTAGCAGAGG8210.1957581754670418No Hit
GTTGCGCGGCGGACGAGTACGTACCGGAACGCGCAGACCGCTCA8190.19528129806030115No Hit
GATACCGTCGGTCGGAACGAACGGCGTGCCTTTTTCGAAATATT7990.19051252399289453No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT7610.18145185326482194No Hit
CTTTGGGTGTTTCTGTTTCTGACGCGCTTCGCTAGAGGTACGTT7360.17549088568056367No Hit
GCCCTCAACATCCAGCATTTCCATGTCGCCCAGGGTAAACTTAG7150.17048367290978672No Hit
GTTACAGGTTACAGTCAGCGCTTTGTTAACATCAGAACCGGTCA7020.16738396976597242No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA6830.16285363440193612No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT6810.16237675699519546No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA6700.15975393125812182No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT6450.15379296367386355No Hit
CTGTGCGCCCTACGCGGAGGTGGCCTATTCATCGGTGCAGGATT6050.1442554155390503No Hit
GATAACGAAGCTAGCGGAGGTCGGGGTAATGACCACAGAGGACG5820.1387713253615327No Hit
GTAGTCATAGTCACGCGGCAGCAGTACACGTGCGGTAACACGCT5810.13853288665816235No Hit
AAAGCCATCCTGTTTAGCTTTATTGATGATGTCCTTGATGGAGT5730.1366253770311997No Hit
TGGGATCTGCAGAGTCTGGTTCGGCATGATCAGATAACCACGCA5630.1342409899974964No Hit
GGCAATATTTAAATATGCTCGTCAGGTGGACGGACCTTCGCCCG5200.12398812575257216No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG5180.12351124834583148No Hit
GCCGTTACCAGCGTACAGCAGAGACACTTTTTCCGGTTCGATAC5120.12208061612560951No Hit
GGTGGACGGGCCTTCGCCCGGGTCGTCAGCCGGACCCTGCTCGA5070.12088842260875785No Hit
GTCGCCATACAGCAGGGAAACTTTTTCCGGCTCGATGTTGGAGT4920.1173118420582029No Hit
GTTCATCAGGCTAGAGAAGATGGTTGCAGAGCGGTAACCATACA4300.10252864244924237No Hit
GTCCACAATTTTGCTCGCAGCAGATGGGTCGGCGATTTTATTCA4290.10229020374587204No Hit
GCCCGCACCTTCTTCTTCTTCCTCTTCCTCCAGGCCGATGCTAG4240.10109801022902037No Hit
AACACCGTTGGTGGTACCAACACCTTTGTCCGGAGATTTGGATG4230.10085957152565005No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG4210.10038269411890939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)