FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007938102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419395
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCCAGGGTCGTCGTGGTAACGTTTACT71201.697683567996757No Hit
CGTCGCCACACCCAGAAAGCACCGAAACGTA46991.1204234671371858No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC24690.5887051586213474No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA21940.5231345151945064No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21540.5135969670596932No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA20640.4921374837563633No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG19270.45947138139462795No Hit
GAACCGATCTACGCTACCATCGACGACCTGG18310.4365812658710762No Hit
CCGGATTTTGAACAGAACATTGCGACCACCA16310.38889352519701No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA16300.3886550864936396No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13720.32713790102409424No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT13010.3102087530848007No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA12970.30925499827131936No Hit
GTGAACGCAAAAATTGATCGCCTGAACCAGA11330.2701510509185851No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG9880.2355774389298871No Hit
CAGGCCCCGAAAGCTGACGCGCAACAGAACA9630.22961647134562882No Hit
CTGCCGATCCAGCAGGTCAGCAAAATGGGCG9420.22460925857485187No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG9330.22246331024451887No Hit
ACTAGCCCGGAAGAACCGATCTACACCCAGG8680.20696479452544736No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC8650.2062494784153364No Hit
AACACCGATGCACACGCTTCCAGCGATGCCC8180.19504285935693083No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG7490.17859058882437798No Hit
CCGCCGCGCACCCCTACCTGGGAATCCCCGG7460.17787527271426698No Hit
TTCAACAAAAAACAGAGCTCCGACGTAAAAG7300.17406025346034168No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG7260.17310649864686037No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC6850.16333051180867678No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG6750.16094612477497347No Hit
ACTGCACTGTTCGCTCGCGAATACCAGACCC6120.1459244864626426No Hit
GAACGTGGTAACTTCTCCAAATTCACCCTGG5800.138294447954792No Hit
AAAGTGTACAGCCAGAACGGTCTGGTCCTGC5740.13686381573457004No Hit
ACCGATTGCGACCTGGATCCGATGGAAGGCA5740.13686381573457004No Hit
GTGGGCGGCTCTGATGCACAGAACACCTCTA5590.13328723518401506No Hit
CCTCGTCGTCCGCCGCCAGGCCGTCGCCCGT5570.1328103577772744No Hit
TCCCCGCCTCGTCGCCCGCCGCCGGGTCGTC5460.13018753204020078No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5410.1289953385233491No Hit
ACCCTGGGCGACATGGAAATGCTGGATGCAG4980.11874247427842487No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA4800.11445057761775891No Hit
ACCGGCCTGGGTGTCACTTCCCGTGTGGCTT4430.10562834559305666No Hit
CCGCAGCTGCAGACTACCGGTCGTCCGTCTC4310.1027670811526127No Hit
TCTACCGAAAGCGTTGACAAAAACTTTGTTG4220.10062113282227972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)