FastQCFastQC Report
Wed 31 May 2023
EGAF00007938104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007938104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412155
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57911.4050539238878577No Hit
ATGACCCAGGGTCGTCGTGGTAACGTTTACT44101.0699858063107326No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT12820.3110480280477005No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC9500.2304958086156907No Hit
ATGATGGACCCGAACTCTACCAGCGAAGACG9340.22661377394426857No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG8870.21521029709696596No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG8680.21060038092465214No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA8650.20987249942376046No Hit
TCTGCCGCCCACTTTCTGCCGCAACAGCCGA8480.2057478375853744No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG7670.18609503706129976No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC7640.18536715556040811No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG7350.17833096771845544No Hit
GGTCGTCAGGGCTCCGATGTCGAACTGGAAT6990.16959638970775556No Hit
ATCGAACAGAAACAGCTGCTGCAGGGCGATG6720.16304545619973068No Hit
TCTCACGAAGAACAGCCGCGTTACGAAGATC6550.15892079436134463No Hit
GGTCGTCAGGGCAGCGACGTAGAACTGGAAT6410.15552401402385024No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6060.14703206318011428No Hit
TCTCACGAAGAACAACCGCGTTATGAGGATC5910.143392655675656No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5760.13975324817119772No Hit
CAGAAACAGCTGCTGCAGGGCGATGTGGAAG5620.13635646783370334No Hit
CAGGGCGCGCTGACCCTGTCCCTGCCGAAAC5400.13101867016049787No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC5130.12446773665247299No Hit
GCTGGTATCGTGACCGTCCCGCCACGCTCCC5110.12398248231854521No Hit
CAGGACTCTCTGCCGGACACTAAAGCCTCTG5010.12155621064890636No Hit
TCTGCGTCCGCCTCCGTTCTGTCTCCGACCG4990.12107095631497858No Hit
ACCGCATCCGGTGAAGAAGTTGCGGTACTGA4880.11840205747837586No Hit
ACCGCATCCGGTGAAGAAGTAGCTGTTCTGT4850.1176741759774842No Hit
GCGGAAGTGAATGTTGACCAGGCTCACCTGG4820.11694629447659255No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG4700.11403476847302592No Hit
TCCGCGCCGCCGTCCCCTGTCAAAGGTCGTG4660.11306425980517039No Hit
TCTGGCGCTGGCACCTCTTCTGCCTCTTCCG4660.11306425980517039No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA4650.1128216326382065No Hit
TCCCGCGTTCCGGCTAAAAAACCGCGTAAAC4490.10893959796678433No Hit
ACCGAACCTACTACTGATTACGGCGGTGACT4460.1082117164658927No Hit
TCTCGCGTGCCGGCGAAGAAACCGCGTAAAC4290.10408705462750664No Hit
TCTGGTGACGAAGATAGCGACTCCTCCGATG4250.1031165459596511No Hit
GGTCGTCAGGGCTCCGACGTCGAGCTGGAAA4220.10238866445875945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)